Development of a bispecific monoclonal antibody as a universal immunoprobe for detecting biotinylated macromolecules

被引:12
作者
Cao, Y [1 ]
Christian, S [1 ]
Suresh, MR [1 ]
机构
[1] Univ Alberta, Fac Pharm & Pharmaceut Sci, Edmonton, AB T6G 2N8, Canada
基金
加拿大自然科学与工程研究理事会;
关键词
micro-electrofusion; bsMab; biotin;
D O I
10.1016/S0022-1759(98)00154-9
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Bispecific monoclonal antibody (BsMab) combining two different antigen binding sites, anti-biotin and anti-HRPO paratopes, could be used as a universal immunoprobe for detecting all biotinylated macromolecules. First, a mouse hybridoma cell line secreting monospecific anti-biotin Mab was generated and characterized. Second, a quadroma cell line which could continuously secrete bsMab (anti-biotin x anti-HRPO) was developed by a nonselective microelectrofusion method. The supernatant containing bsMab was collected from tissue culture medium and purified with two affinity columns. This bsMab has comparable avidity to commercial streptavidin-HRPO when tested against biotinylated macromolecules. Compared to streptavidin, this bsMab can bind the enzyme and thus eliminate the need for chemical conjugation. This bsMab can be used as a promising immunoprobe for detecting many macromolecules bearing biotin markers, such as protein, phage, liposome and DNA in different bioassay systems. (C) 1998 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:85 / 91
页数:7
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