Development of a bispecific monoclonal antibody as a universal immunoprobe for detecting biotinylated macromolecules

被引：12

作者：

Cao, Y ^{[1
]}

Christian, S ^{[1
]}

Suresh, MR ^{[1
]}

机构：

[1] Univ Alberta, Fac Pharm & Pharmaceut Sci, Edmonton, AB T6G 2N8, Canada

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1998年 / 220卷 / 1-2期

基金：

加拿大自然科学与工程研究理事会;

关键词：

micro-electrofusion; bsMab; biotin;

D O I：

10.1016/S0022-1759(98)00154-9

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Bispecific monoclonal antibody (BsMab) combining two different antigen binding sites, anti-biotin and anti-HRPO paratopes, could be used as a universal immunoprobe for detecting all biotinylated macromolecules. First, a mouse hybridoma cell line secreting monospecific anti-biotin Mab was generated and characterized. Second, a quadroma cell line which could continuously secrete bsMab (anti-biotin x anti-HRPO) was developed by a nonselective microelectrofusion method. The supernatant containing bsMab was collected from tissue culture medium and purified with two affinity columns. This bsMab has comparable avidity to commercial streptavidin-HRPO when tested against biotinylated macromolecules. Compared to streptavidin, this bsMab can bind the enzyme and thus eliminate the need for chemical conjugation. This bsMab can be used as a promising immunoprobe for detecting many macromolecules bearing biotin markers, such as protein, phage, liposome and DNA in different bioassay systems. (C) 1998 Elsevier Science B.V. All rights reserved.

引用

页码：85 / 91

页数：7