Strongylus asini (Nematoda, Strongyloidea): Genetic relationships with other Strongylus species determined by ribosomal DNA

被引：41

作者：

Hung, GC

Jacobs, DE

Krecek, RC

Gasser, RB

Chilton, NB

机构：

[1] UNIV LONDON ROYAL VET COLL,DEPT PATHOL & INFECT DIS,HATFIELD AL9 7TA,HERTS,ENGLAND

[2] UNIV MELBOURNE,DEPT VET SCI,WERRIBEE,VIC 3030,AUSTRALIA

[3] UNIV PRETORIA,DEPT VET TROP DIS,ZA-0110 ONDERSTEPOORT,SOUTH AFRICA

来源：

INTERNATIONAL JOURNAL FOR PARASITOLOGY | 1996年 / 26卷 / 12期

关键词：

Strongylus asini; S-edentatus; S-equinus; S-vulgaris; equidae; horse; zebra; rDNA; second internal transcribed spacer; PCR-linked RFLP;

D O I：

10.1016/S0020-7519(96)00136-1

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Genomic DNA was isolated from adult Strongylus asini collected from zebra. The second ribosomal transcribed spacer (ITS-2) was amplified and sequenced using polymerase chain reaction (PCR) based techniques. The DNA sequence was compared with previously published data for 3 related Strongylus species. A PCR-linked restriction fragment length polymorphism method allowed the 4 species to be differentiated unequivocally. The ITS-2 sequence of S. asini was found to be more similar to those of S. edentatus (87.1%) and S. equinus (95.3%) than to that of S. vulgaris (73.9%). This result confirms that S. asini and S. vulgaris represent separate species and supports the retention of the 4 species within 1 genus. Copyright (C) 1996 Australian Society for Parasitology. Published by Elsevier Science Ltd.

引用

页码：1407 / 1411

页数：5

共 14 条

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