GENETIC-MARKERS FOR CLOSELY-RELATED PARASITIC NEMATODES

被引：69

作者：

GASSER, RB ^{[1
]}

HOSTE, H ^{[1
]}

机构：

[1] INRA,PATHOL AVIAIRE & PARASITOL STN,CTR TOURS,F-37380 NOUZILLY,FRANCE

来源：

MOLECULAR AND CELLULAR PROBES | 1995年 / 9卷 / 05期

基金：

澳大利亚研究理事会;

关键词：

RIBOSOMAL DNA; INTERNAL TRANSCRIBED SPACER; PCR-RFLP; GENETIC MARKERS; PARASITIC NEMATODES; SPECIES IDENTIFICATION; DIFFERENTIATION;

D O I：

10.1016/S0890-8508(95)91588-5

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Seven species of closely-related nematode parasite (Trichostrongylus axei, T. colubriformis, T. probolurus, T. retortaeformis, T. rugatus, T. vitrinus and T. tenuis) were characterized using a polymerase chain reaction-linked restriction fragment length polymorphism technique (PCR-RFLP). The rDNA region spanning the first and second internal transcribed spacers as well as the 5.8S rDNA gene (ITS+) was amplified from isolates of each of the seven species, digested separately with six restriction endonucleases (Dra I, Hinf I, Rsa I, Vsp I, Nla III and Tsp 509 I) and the fragments separated by agarose gel electrophoresis. PCR-RFLP of ITS+ produced characteristic patterns for each Trichostrongylus species examined. No variation in RFLP patterns was observed among different isolates for species where multiple isolates were examined. The present study demonstrates that the ITS+ provides genetic markers for the species identification of closely-related parasitic nematodes, and indicates the usefulness of these markers for diagnostic purposes, and epidemiologic and molecular-systematic studies on parasites and other eukaryotic organisms. (C) 1995 Academic Press Limited

引用

页码：315 / 319

页数：5

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