Death rate in a small air-lift loop reactor of Vero cells grown on solid microcarriers and in macroporous microcarriers

被引：10

作者：

Martens, DE ^{[1
]}

Nollen, EAA ^{[1
]}

Hardeveld, M ^{[1
]}

vanderVeldendeGroot, CAM ^{[1
]}

deGooijer, CD ^{[1
]}

Beuvery, EC ^{[1
]}

Tramper, J ^{[1
]}

机构：

[1] NATL INST PUBL HLTH & ENVIRONM PROTECT, LAB INACTIVATED VIRAL VACCINES, NL-3720 BA BILTHOVEN, NETHERLANDS

来源：

CYTOTECHNOLOGY | 1996年 / 21卷 / 01期

关键词：

air lift; macroporous; microcarrier; shear; sparging; Vero cell;

D O I：

10.1007/BF00364836

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The death rate of Vero cells grown on Cytodex-3 microcarriers was studied as a function of the gas flow rate in a small ah-lift loop reactor. The death rate may be described by first-order death-rate kinetics. The first-order death-rate constant as calculated from the decrease in viable cells, the increase in dead cells and the increase in LDH activity is linear proportional to the gas flow rate, with a specific hypothetical killing volume in which all cells are killed of about 2 . 10(-3) m(3) liquid per m(3) of air bubbles. In addition, an experiment was conducted in the same air-lift reactor with Vero cells grown inside porous Asahi microcarriers. The specific hypothetical killing volume calculated from this experiment has a value of 3 . 10(-4) m(3) liquid per m(3) of air bubbles, which shows that the porous microcarriers were at least in part able to protect the cells against the detrimental hydrodynamic forces generated by the bubbles.

引用

页码：45 / 59

页数：15

共 37 条

[1]

[Anonymous], 1989, AIRLIFT BIOREACTORS

[2] MICROSCOPIC VISUALIZATION OF INSECT CELL BUBBLE INTERACTIONS .1. RISING BUBBLES, AIR MEDIUM INTERFACE, AND THE FOAM LAYER [J].