Alternate splice-site utilization in the gene for the catalytic subunit of the DNA-activated protein kinase, DNA-PKcs

被引：20

作者：

Connelly, MA ^{[1
]}

Zhang, H ^{[1
]}

Kieleczawa, J ^{[1
]}

Anderson, CW ^{[1
]}

机构：

[1] BROOKHAVEN NATL LAB,DEPT BIOL,UPTON,NY 11973

来源：

GENE | 1996年 / 175卷 / 1-2期

关键词：

DNA double-strand break repair; V(D)J recombination; phosphatidylinositol kinase-related protein;

D O I：

10.1016/0378-1119(96)00135-7

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Analysis of polyA-selected RNAs and cDNA clones from several human cell lines revealed the presence of a 93-bp exon in the PI kinase homology region of DNA-PKcs that was not present in the cDNA sequence derived from HeLa cell cDNA clones. RT-PCR showed that most DNA-PKcs mRNAs in human cells have this exon. Thus, the nascent DNA-PKcs, polypeptide is composed of 4127 aa and has a predicted mel. wt. of 469 021 (470 kDa).

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收藏

页码：271 / 273

页数：3

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