Tethering the Z ring to the membrane through a conserved membrane targeting sequence in FtsA

被引:321
作者
Pichoff, S [1 ]
Lutkenhaus, J [1 ]
机构
[1] Univ Kansas, Med Ctr, Dept Microbiol Mol Genet & Immunol, Kansas City, KS 66160 USA
关键词
D O I
10.1111/j.1365-2958.2005.04522.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The cytokinetic Z ring is required for bacterial cell division. It consists of polymers of FtsZ, the bacterial ancestor of eukaryotic tubulin, linked to the cytoplasmic membrane. Formation of a Z ring in Escherichia coli occurs as long as one of two proteins, ZipA or FtsA, is present. Both of these proteins bind FtsZ suggesting that they might function to tether FtsZ filaments to the membrane. Although ZipA has a transmembrane domain and therefore can function as a membrane anchor, interaction of FtsA with the membrane has not been explored. In this study we demonstrate that FtsA, which is structurally related to eukaryotic actin, has a conserved C-terminal amphipathic helix that is essential for FtsA function. It is required to target FtsA to the membrane and subsequently to the Z ring. As FtsA is much more widely conserved in bacteria than ZipA, it is likely that FtsA serves as the principal membrane anchor for the Z ring.
引用
收藏
页码:1722 / 1734
页数:13
相关论文
共 62 条
[51]   FtsZ exhibits rapid movement and oscillation waves in helix-like patterns in Escherichia coli [J].
Thanedar, S ;
Margolin, W .
CURRENT BIOLOGY, 2004, 14 (13) :1167-1173
[52]   Prokaryotic origin of the actin cytoskeleton [J].
van den Ent, F ;
Amos, LA ;
Löwe, J .
NATURE, 2001, 413 (6851) :39-44
[53]   Crystal structure of the cell division protein FtsA from Thermotoga maritima [J].
van den Ent, F ;
Löwe, J .
EMBO JOURNAL, 2000, 19 (20) :5300-5307
[54]   ARC6 is a J-domain plastid division protein and an evolutionary descendant of the cyanobacterial cell division protein Ftn2 [J].
Vitha, S ;
Froehlich, JE ;
Koksharova, O ;
Pyke, KA ;
van Erp, H ;
Osteryoung, KW .
PLANT CELL, 2003, 15 (08) :1918-1933
[55]  
Wang Xunde, 1997, Journal of Bacteriology, V179, P5551
[56]   Localization of FtsI (PBP3) to the septal ring requires its membrane anchor, the Z ring, FtsA, FtsQ, and FtsL [J].
Weiss, DS ;
Chen, JC ;
Ghigo, JM ;
Boyd, D ;
Beckwith, J .
JOURNAL OF BACTERIOLOGY, 1999, 181 (02) :508-520
[57]   Coordination of cell division and chromosome segregation by a nucleoid occlusion protein in Bacillus subtillis [J].
Wu, LJ ;
Errington, J .
CELL, 2004, 117 (07) :915-925
[58]   Role of the carboxy terminus of Escherichia coli FtsA in self-interaction and cell division [J].
Yim, L ;
Vandenbussche, G ;
Mingorance, J ;
Rueda, S ;
Casanova, M ;
Ruysschaert, JM ;
Vicente, M .
JOURNAL OF BACTERIOLOGY, 2000, 182 (22) :6366-6373
[59]   FtsZ ring clusters in min and partition mutants: role of both the Min system and the nucleoid in regulating FtsZ ring localization [J].
Yu, XC ;
Margolin, W .
MOLECULAR MICROBIOLOGY, 1999, 32 (02) :315-326
[60]   Membrane binding by MinD involves insertion of hydrophobic residues within the C-terminal amphipathic helix into the bilayer [J].
Zhou, H ;
Lutkenhaus, J .
JOURNAL OF BACTERIOLOGY, 2003, 185 (15) :4326-4335