Exploring the active site of human factor Xa protein by NMR screening of small molecule probes

被引:15
作者
Fielding, L [1 ]
Fletcher, D
Rutherford, S
Kaur, J
Mestres, J
机构
[1] Organ Labs Ltd, Dept Analyt Chem, Newhouse ML1 5SH, Lanark, Scotland
[2] Organ Labs Ltd, Dept Med Chem, Newhouse ML1 5SH, Lanark, Scotland
关键词
D O I
10.1039/b310265c
中图分类号
O62 [有机化学];
学科分类号
070303 ; 081704 ;
摘要
A collection of small molecules (MW < 350 Da) was screened for binding to human factor Xa using saturation transfer difference NMR spectroscopy to detect binding. The NMR screening experiments identified four hits. Binding isotherms constructed from NMR linewidth data showed that the binding affinities of the hits were all in the 30-210 mu M range. Competition binding experiments showed that three of the ligands were displaced by a known mu M inhibitor of factor Xa. The success of the method for identifying new ligands and the relevance of this information to the design of new factor Xa inhibitors are discussed.
引用
收藏
页码:4235 / 4241
页数:7
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