Partial characterization of serotonin N-acetyltransferases from northern pike (Esox lucius, L) pineal organ and retina: Effects of temperature

In vertebrates; the nocturnal rise in pineal organ and retinal melatonin synthesis results from the increase in the activity of the serotonin N-acetyltransferase (NAT), a cAMP-dependent enzyme. In the Gs pineal organ in culture, light and temperature act in a similar manner on cAMP content and NAT activity, It is not known whether the effects of temperature are mediated through cAMP or through modifications of NAT kinetics. The present study was designed: (1) to find out whether NAT activity from pineal organ homogenates is similar to NAT activity from pineal organs in culture, with regard to variations in temperature, and (2) to compare NAT activity from the pineal organ and the retina, Pineal organ and retinal NAT activity increased linearly with protein concentrations, Higher activities were obtained with 0.2 mol/l of phosphate buffer, pH 6. Higher molarity or a higher PH induced a decrease in retinal and pineal organ NAT activity: retinal NAT was more sensitive than pineal organ NAT to changes in molarity, whereas the opposite held true as far as pH was concerned. Pineal organ and retinal NAT obeyed ce Michaelis-Menten equation with respect to increasing concentrations of acetyl-coenzyme A, With increasing concentrations of tryptamine: (1) pineal organ NAT activity increased in a manner suggesting positive co-operativity, (2) retinal NAT displayed, after an initial increase, inhibition by substrate The kinetics of the reactions were temperature dependent. Maximal activities were reached at 18/20 degrees C in the pineal organ and al 37 degrees C in the retina. The present study is the first to describe the optimum conditions fur the assay of NAT activity in homogenates from the retina of fish and from the pineal organ of poikilotherms, and also the first to compare some characteristics of MAT activity from these two analogous organs;Our results suggest that the effects of temperature on melatonin production are mediated, at least in part, through modifications of NAT kinetics. Future studies will aim to clarify whether the activities measured in the pineal organ and retinal homogenates reflect the presence of one or of several enzymes.