Microfluidic droplets: new integrated workflows for biological experiments

被引：132

作者：

Kintses, Balint ^{[1
]}

van Vliet, Liisa D. ^{[1
]}

Devenish, Sean R. A. ^{[1
]}

Hollfelder, Florian ^{[1
]}

机构：

[1] Univ Cambridge, Dept Biochem, Cambridge CB2 1GA, England

来源：

CURRENT OPINION IN CHEMICAL BIOLOGY | 2010年 / 14卷 / 05期

基金：

英国工程与自然科学研究理事会;

关键词：

POLYMERASE-CHAIN-REACTION; SINGLE-CELLS; PICOLITER DROPLETS; DIRECTED EVOLUTION; PROTEIN EXPRESSION; REAL-TIME; ON-CHIP; MICRODROPLETS; AMPLIFICATION; ENCAPSULATION;

D O I：

10.1016/j.cbpa.2010.08.013

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Miniaturization of the classical test tube to picoliter dimensions is possible in monodisperse water-in-oil droplets that are generated in microfluidic devices. The establishment of standard unit operations for droplet handling and the ability to carry out experiments with DNA, proteins, cells and organisms provides the basis for the design of more complex workflows to address biological challenges. The emerging experimental format makes possible a quantitative readout for large numbers of experiments with a precision comparable to the macroscopic scale. Directed evolution, diagnostics and compound screening are areas in which the first steps are being taken toward the long-term goal of transforming the way we design and carry out experiments.

引用

页码：548 / 555

页数：8