Integrating 3D Printing and Biomimetic Mineralization for Personalized Enhanced Osteogenesis, Angiogenesis, and Osteointegration

被引：113

作者：

Ma, Limin ^{[1
,2
,5
]}

Wang, Xiaolan ^{[1
]}

Zhao, Naru ^{[2
]}

Zhu, Ye ^{[3
]}

Qiu, Zhiye ^{[4
]}

Li, Qingtao ^{[2
]}

Zhou, Ye ^{[5
]}

Lin, Zefeng ^{[5
]}

Li, Xiang ^{[6
]}

Zeng, Xiaolong ^{[1
]}

Xia, Hong ^{[5
]}

Zhong, Shizhen ^{[7
]}

Zhang, Yu ^{[1
]}

Wang, Yingjun ^{[2
]}

Mao, Chuanbin ^{[3
,8
]}

机构：

[1] Guangdong Acad Med Sci, Guangdong Gen Hosp, Dept Orthoped, Guangzhou 510080, Guangdong, Peoples R China

[2] South China Univ Technol, Sch Mat Sci & Engn, Guangzhou 510641, Guangdong, Peoples R China

[3] Univ Oklahoma, Inst Biomed Engn Sci & Technol, Stephenson Life Sci Res Ctr, Dept Chem & Biochem, Norman, OK 73072 USA

[4] Tsinghua Univ, Inst Regenerat Med & Biomimet Mat, Beijing 100084, Peoples R China

[5] Guangzhou Gen Hosp Guangzhou Mil Command, Guangdong Key Lab Orthoped Technol & Implant, Dept Orthoped, Guangzhou 510010, Guangdong, Peoples R China

[6] Shanghai Jiao Tong Univ, State Key Lab Mech Syst & Vibrat, Sch Mech Engn, Shanghai 200240, Peoples R China

[7] Southern Med Univ, Sch Basic Med Sci, Guangzhou 510515, Guangdong, Peoples R China

[8] Zhejiang Univ, Sch Mat Sci & Engn, Hangzhou 310027, Zhejiang, Peoples R China

来源：

ACS APPLIED MATERIALS & INTERFACES | 2018年 / 10卷 / 49期

基金：

美国国家卫生研究院; 中国国家自然科学基金;

关键词：

porous titanium alloys; mineralized collagen; angiogenesis; osteogenesis; osteointegration; POROUS METALS; GROWTH; SCAFFOLDS; TI6AL4V; BIOCOMPATIBILITY; OSSEOINTEGRATION; FABRICATION; COMPOSITE; PHAGE; STEM;

D O I：

10.1021/acsami.8b17495

中图分类号：

TB3 [工程材料学];

学科分类号：

082905 [生物质能源与材料];

摘要：

Titanium (Ti) alloy implants can repair bone defects at load-bearing sites. However, they mechanically mismatch with the natural bone and lack customized adaption with the irregularly major-sized load-bearing bone defects, resulting in the failure of implant fixation. Mineralized collagen (MC), a building block in bone, can induce angiogenesis and osteogenesis, and 3D printing technology can be employed to prepare scaffolds with an overall shape customized to the bone defect. Hence, we induced the formation of MC, made of hydroxyapatite (HAp) nanocrystals and collagen fibers, in 3D-printed porous Ti6Al4V (PT) scaffolds through in situ biomimetic mineralization. The resultant MC/PT scaffolds exhibited a bone-like Young's modulus and were customized to the anatomical contour of actual bone defects of rabbit model. We found that the biocompatibility and osteogenic differentiation are best when the mass ratio between HAp nanocrystals and collagen fibers is 1 in MC. We then implanted the MC/PT scaffolds into the customized radius defect rabbit model and found that the MC/PT scaffolds significantly improved the vascularized bone tissue formation and integration between new bone and the implants. Therefore, a combination of 3D printing and biomimetic mineralization could lead to customized 3D PT scaffolds for enhanced angiogenesis, osteogenesis, and osteointegration. Such scaffolds represent novel patient-specific implants for precisely repairing irregular major-sized load-bearing bone defects.

引用

页码：42146 / 42154

页数：9

共 37 条

[1]

Fully Porous 3D Printed Titanium Femoral Stem to Reduce Stress-Shielding Following Total Hip Arthroplasty [J].