Identification and structural characterization of nucleus-encoded transfer RNAs imported into wheat mitochondria

被引:44
作者
Glover, KE [1 ]
Spencer, DF [1 ]
Gray, MW [1 ]
机构
[1] Dalhousie Univ, Dept Biochem & Mol Biol, Halifax, NS B3H 4H7, Canada
关键词
D O I
10.1074/jbc.M007708200
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Despite its large size (200-2400 kilobase pairs), the mitochondrial genome of angiosperms does not encode the minimal set of tRNAs required to support mitochondrial protein synthesis. Here we report the identification of cytosolic-like tRNAs in wheat mitochondria using a method involving quantitative hybridization to distinguish among three tRNA classes: (i) those encoded by mitochondrial DNA (mtDNA) and localized in mitochondria, (ii) those encoded by nuclear DNA and located in the cytosol, and (iii) those encoded by nuclear DNA and found in both the cytosol and mitochondria. The latter class comprises tRNA species that are considered to be imported into mitochondria to compensate for the deficiency of mtDNA-encoded tRNAs, In a comprehensive survey of the wheat mitochondrial tRNA population, we identified 14 such imported tRNAs, the structural characterization of which is presented here. These imported tRNAs complement 16 mtDNA-encoded tRNAs, for a total of at least 30 distinct tRNA species in wheat mitochondria. Considering differences in the set of mtDNA-encoded and imported tRNAs in the mitochondria of various land plants, the import system must be able to adapt relatively rapidly over evolutionary time with regard to the particular cytosolic-like tRNA that are brought into mitochondria.
引用
收藏
页码:639 / 648
页数:10
相关论文
共 57 条
[1]   Occurrence of nuclear-encoded tRNA(IIe) in mitochondria of the liverwort Marchantia polymorpha [J].
Akashi, K ;
Sakurai, K ;
Hirayama, J ;
Fukuzawa, H ;
Ohyama, K .
CURRENT GENETICS, 1996, 30 (02) :181-185
[2]   Coexistence of nuclear DNA-encoded tRNAVal(AAC) and mitochondrial DNA-encoded tRNAVal(UAC) in mitochondria of a liverwort Marchantia polymorpha [J].
Akashi, K ;
Takenaka, M ;
Yamaoka, S ;
Suyama, Y ;
Fukuzawa, H ;
Ohyama, K .
NUCLEIC ACIDS RESEARCH, 1998, 26 (09) :2168-2172
[3]   Accumulation of nuclear-encoded tRNA(Thr)(AGU) in mitochondria of the liverwort Marchantia polymorpha [J].
Akashi, K ;
Hirayama, J ;
Takenaka, M ;
Yamaoka, S ;
Suyama, Y ;
Fukuzawa, H ;
Ohyama, K .
BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION, 1997, 1350 (03) :262-266
[4]   Gapped BLAST and PSI-BLAST: a new generation of protein database search programs [J].
Altschul, SF ;
Madden, TL ;
Schaffer, AA ;
Zhang, JH ;
Zhang, Z ;
Miller, W ;
Lipman, DJ .
NUCLEIC ACIDS RESEARCH, 1997, 25 (17) :3389-3402
[5]   THE NUCLEOTIDE-SEQUENCE OF TRANSFER RNASER AND TRANSFER RNAILE OF YELLOW LUPIN SEEDS [J].
BARCISZEWSKA, M ;
KEITH, G ;
DIRHEIMER, G ;
BARCISZEWSKI, J .
NUCLEIC ACIDS RESEARCH, 1988, 16 (16) :8175-8175
[6]   THE NUCLEOTIDE-SEQUENCE OF A VALINE ACCEPTING TRANSFER-RNA FROM LUPINUS-LUTEUS (LUPIN) SEEDS [J].
BARCISZEWSKA, M ;
JONES, DS .
NUCLEIC ACIDS RESEARCH, 1987, 15 (03) :1333-1333
[7]   THE PRIMARY STRUCTURE OF WHEAT-GERM TRANSFER RNAARG - THE SUBSTRATE FOR ARGINYL-TRANSFER RNAARG-PROTEIN TRANSFERASE [J].
BARCISZEWSKA, MZ ;
KEITH, G ;
KUBLI, E ;
BARCISZEWSKI, J .
BIOCHIMIE, 1986, 68 (02) :319-323
[8]   THE PRIMARY STRUCTURE OF THE 1ST PLANT TRANSFER RNAHIS [J].
BARCISZEWSKA, MZ ;
KEITH, G ;
DIRHEIMER, G ;
BARCISZEWSKI, J .
PLANT SCIENCE, 1986, 47 (02) :103-107
[9]   Wheat cytoplasmic arginine tRNA isoacceptor with a U*CG anticodon is an efficient UGA suppressor in vitro [J].
Baum, M ;
Beier, H .
NUCLEIC ACIDS RESEARCH, 1998, 26 (06) :1390-1395
[10]   UAG READTHROUGH DURING TMV RNA TRANSLATION - ISOLATION AND SEQUENCE OF 2 TRANSFER RNASTYR WITH SUPPRESSOR ACTIVITY FROM TOBACCO PLANTS [J].
BEIER, H ;
BARCISZEWSKA, M ;
KRUPP, G ;
MITNACHT, R ;
GROSS, HJ .
EMBO JOURNAL, 1984, 3 (02) :351-356