Coexistence of nuclear DNA-encoded tRNAVal(AAC) and mitochondrial DNA-encoded tRNAVal(UAC) in mitochondria of a liverwort Marchantia polymorpha

被引:22
作者
Akashi, K [1 ]
Takenaka, M [1 ]
Yamaoka, S [1 ]
Suyama, Y [1 ]
Fukuzawa, H [1 ]
Ohyama, K [1 ]
机构
[1] Kyoto Univ, Grad Sch Agr, Div Appl Life Sci, Lab Plant Mol Biol,Sakyo Ku, Kyoto 6068502, Japan
关键词
D O I
10.1093/nar/26.9.2168
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The liverwort Marchantia polymorpha mitochondrial DNA encodes almost all tRNAs required for mitochondrial translation except for the isoleucine (AUU, AUC) and threonine (ACA, ACG) codons, while the missing tRNAs are supplied in part by the nucleus and imported in mitochondria, In this paper, we report a finding of two radically different nuclear tRNA(Val)(AAC) genes and import of the corresponding tRNA iso-acceptors in M.polymorpha mitochondria. This finding is surprising since the mtDNA encodes the gene for tRNA(Val)(UAC), which alone was considered sufficient for translating all four valine codons GUN by the U/N wobble mechanism. The present results suggest for the first time that the import of ncDNA-encoded tRNAs may result in decoding overlaps in plant mitochondria. The coexistence of nuclear DNA-encoded tRNA(Val)(AAC) and mitochondrial DNA-encoded tRNA(Val)(UAC) in liverwort mitochondria and the significance for the decoding mechanism as well as evolution of tRNA import are discussed.
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页码:2168 / 2172
页数:5
相关论文
共 33 条
[1]   Occurrence of nuclear-encoded tRNA(IIe) in mitochondria of the liverwort Marchantia polymorpha [J].
Akashi, K ;
Sakurai, K ;
Hirayama, J ;
Fukuzawa, H ;
Ohyama, K .
CURRENT GENETICS, 1996, 30 (02) :181-185
[2]   Accumulation of nuclear-encoded tRNA(Thr)(AGU) in mitochondria of the liverwort Marchantia polymorpha [J].
Akashi, K ;
Hirayama, J ;
Takenaka, M ;
Yamaoka, S ;
Suyama, Y ;
Fukuzawa, H ;
Ohyama, K .
BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION, 1997, 1350 (03) :262-266
[3]   UAG READTHROUGH DURING TMV RNA TRANSLATION - ISOLATION AND SEQUENCE OF 2 TRANSFER RNASTYR WITH SUPPRESSOR ACTIVITY FROM TOBACCO PLANTS [J].
BEIER, H ;
BARCISZEWSKA, M ;
KRUPP, G ;
MITNACHT, R ;
GROSS, HJ .
EMBO JOURNAL, 1984, 3 (02) :351-356
[4]   NATIVE AND IMPORTED TRANSFER-RNA IN MITOCHONDRIA [J].
CHIU, N ;
CHIU, A ;
SUYAMA, Y .
JOURNAL OF MOLECULAR BIOLOGY, 1975, 99 (01) :37-50
[5]   GLYCINE CODON DISCRIMINATION AND THE NUCLEOTIDE IN POSITION-32 OF THE ANTICODON LOOP [J].
CLAESSON, C ;
LUSTIG, F ;
BOREN, T ;
SIMONSSON, C ;
BARCISZEWSKA, M ;
LAGERKVIST, U .
JOURNAL OF MOLECULAR BIOLOGY, 1995, 247 (02) :191-196
[6]   CODON-ANTICODON PAIRING - WOBBLE HYPOTHESIS [J].
CRICK, FHC .
JOURNAL OF MOLECULAR BIOLOGY, 1966, 19 (02) :548-&
[7]   A single base change prevents import of cytosolic tRNA(Ala) into mitochondria in transgenic plants [J].
Dietrich, A ;
MarechalDrouard, L ;
Carneiro, V ;
Cosset, A ;
Small, I .
PLANT JOURNAL, 1996, 10 (05) :913-918
[8]   NUCLEAR-ENCODED TRANSFER-RNAS IN PLANT-MITOCHONDRIA [J].
DIETRICH, A ;
WEIL, JH ;
MARECHALDROUARD, L .
ANNUAL REVIEW OF CELL BIOLOGY, 1992, 8 :115-131
[9]   MAPPING ADENINES, GUANINES, AND PYRIMIDINES IN RNA [J].
DONISKELLER, H ;
MAXAM, AM ;
GILBERT, W .
NUCLEIC ACIDS RESEARCH, 1977, 4 (08) :2527-2537
[10]   NUCLEOTIDE-SEQUENCE OF TRANSFER RNAVAL (GUU) FROM ARABIDOPSIS-THALIANA [J].
GOKHMAN, I ;
ZAMIR, A .
NUCLEIC ACIDS RESEARCH, 1990, 18 (22) :6729-6729