Transcription of the Escherichia coli dcw cluster:: Evidence for distal upstream transcripts being involved in the expression of the downstream ftsZ gene

Escherichia coli strains VIP596 and VIP597 have been constructed to compare the amount of transcription of the ftsZ gene derived from proximal promoters in the ddlB-ftsZ region with that originating in the upstream regions of the dcw cluster. Both strains have in common a beta -galactosidase reporter fusion located at the ddlB locus. but differ in that VIP597 has a transcription terminator Omega interposon located downstream from lacZ. In addition, these strains have the ddlB, ftsQ, ftsA and ftsZ genes under the control of the IPTG-inducible promoter P-tac, allowing to control artificially ftsZ expression for normal cell division to take place when beta -galactosidase activity was measured in VIP596 and VIP597 and compared to the levels measured in strain VIP307. in which the lacZ reporter fusion is located in the ftsZ gene. they were found to account for nearly 66% of the total transcription entering into ftsZ This result indicates that the reduction in ftsZ transcription observed when the promoters in the ddlB-ftsA region are disconnected from the upstream sequences of the dcw cluster (as observed by Flardh et al., Mel. Microbiol. 30 (1998) 305-316) in strain VIP490) is the direct consequence of the interruption in the transcription originated upstream and not due to the effect of such sequences on the promoters proximal to ftsZ. (C) 2001 societe francaise de biochimie et biologie moleculaire / Editions scientifiques et medicales Elsevier SAS.