Molecular cloning and sequence analysis of a chicken cDNA encoding tyrosinase-related protein-2/DOPAchrome tautomerase

被引：9

作者：

April, CS ^{[1
]}

Jackson, IJ

Kidson, SH

机构：

[1] Univ Cape Town, Sch Med, Dept Anat & Cell Biol, ZA-7925 Cape Town, South Africa

[2] Western Gen Hosp, MRC, Human Genet Unit, Edinburgh EH4 2XU, Midlothian, Scotland

来源：

GENE | 1998年 / 219卷 / 1-2期

关键词：

DCT; melanocyte; pigment; TYRP2;

D O I：

10.1016/S0378-1119(98)00403-X

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

We have cloned and sequenced a chicken cDNA encoding an L-DOPAchrome tautomerase (DCT) from an embryonic melanocyte cDNA library. The chicken DCT gene encodes a deduced protein of 516 amino acids (aas) and shares 69.2% and 69.9% aa sequence identity with the deduced mouse and human DCT proteins, respectively. Northern blot hybridisation analysis reveals a DCT transcript of 3.5 kb in RNA from the retinal pigment epithelium (RPE) of chick embryos. Genomic Southern blot hybridisation analysis suggests that the chicken DCT gene consists of several introns and spans between 15 and 30 kb of the chicken genome. This study completes the sequencing of all the members of the chicken tyrosinase-related protein gene family and provides evidence that this gene family is conserved between avians and mammals. (C) 1998 Elsevier Science B.V. All rights reserved.

引用

页码：45 / 53

页数：9

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