MicroRNA-519d targets MKi67 and suppresses cell growth in the hepatocellular carcinoma cell line QGY-7703

被引：100

作者：

Hou, Yan-Yan ^{[1
,2
]}

Cao, Wei-Wei ^{[1
,2
]}

Li, Lan ^{[1
,2
]}

Li, Sheng-Ping ^{[3
]}

Liu, Tao ^{[1
,2
]}

Wan, Hai-Ying ^{[1
,2
]}

Liu, Min ^{[1
,2
]}

Li, Xin ^{[1
,2
]}

Tang, Hua ^{[1
,2
]}

机构：

[1] Tianjin Med Univ, Tianjin Life Sci Res Ctr, Tianjin, Peoples R China

[2] Tianjin Med Univ, Basic Med Sch, Tianjin, Peoples R China

[3] Sun Yat Sen Univ Med Sci, Dept Hepatobiliary Oncol, Ctr Canc, Guangzhou, Guangdong, Peoples R China

来源：

CANCER LETTERS | 2011年 / 307卷 / 02期

基金：

中国国家自然科学基金;

关键词：

Hepatocellular carcinoma; MicroRNA; MiR-519d; MKi67; QGY-7703; KI-67; EXPRESSION; KI67; PROLIFERATION; REGIONS; PROTEIN; DIAGNOSIS; PATHWAYS; MARKERS; INDEX;

D O I：

10.1016/j.canlet.2011.04.002

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 [肿瘤学];

摘要：

MicroRNAs (miRNAs) are small non-coding RNAs that regulate the translation of target mRNA transcripts. In this study, we demonstrated that miR-519d was downregulated in human HCC and could suppress growth of the human HCC cell line QGY-7703. Bioinformatics analysis indicated that MKi67 was a putative target of miR-519d. In an EGFP reporter system, we confirmed that MKi67 was a direct target gene of miR-519d. Furthermore, knockdown of MKi67 inhibited QGY-7703 cell growth. These findings indicate that miR-519d targets the MKi67 transcript and suppresses HCC cell growth, suggesting that miR-519d has a tumor suppressive role in human HCC pathogenesis. (C) 2011 Elsevier Ireland Ltd. All rights reserved.

引用

收藏

页码：182 / 190

页数：9

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