Proteomic Analysis of Four Brazilian MON810 Maize Varieties and Their Four Non-Genetically-Modified Isogenic Varieties

被引:29
作者
Balsamo, Geisi M. [1 ]
Cangahuala-Inocente, Gabriela C. [1 ]
Bertoldo, Jean B. [2 ]
Terenzi, Hernan [2 ]
Arisi, Ana C. M. [1 ]
机构
[1] Univ Fed Santa Catarina, Dept Ciencia & Tecnol Alimentos, Ctr Ciencias Agr, BR-88034001 Florianopolis, SC, Brazil
[2] Univ Fed Santa Catarina, Ctr Biol Mol Estrut, BR-88034001 Florianopolis, SC, Brazil
关键词
proteome; profiling techniques; GMO; two-dimensional gel electrophoresis; maize; 2-DIMENSIONAL GEL-ELECTROPHORESIS; COMPARATIVE SAFETY ASSESSMENT; INTERROGATE ALTERATIONS; MODIFIED CROPS; TRANSGENIC MAIZE; QUANTITATION; POTATO; GENE;
D O I
10.1021/jf202635r
中图分类号
S [农业科学];
学科分类号
09 ;
摘要
Profiling techniques have been suggested as a nontargeted approach to detect unintended,effects in genetically modified (GM) plants Seedlings from eight Brazilian maize varieties, four MON810 GM varieties and four non-GM isogenic varieties, were grown under controlled environmental conditions Physiological parameters (aerial part weight, main leaf length, and chlorophyll and total protein contents) were compared, and some differences were observed Nevertheless, these differences were not constant among all GM and non-GM counterparts Leaf proteomic profiles were analyzed using two-dimensional gel electrophoresis (2DE) coupled to mass spectrometry, using six 2DE gels per variety The comparison between MON810 and its counterpart was limited to qualitative differences of fully reproducible protein spot:patterns Twelve exclusive proteins were observed in two of four, maize,variety pairs, all of these leaf proteins were variety specific In this study, MON810 leaf proteomes of four varieties were similar to non-GM counterpart leaf proteomes
引用
收藏
页码:11553 / 11559
页数:7
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