Characterization of a novel structural member, LukE-LukD, of the bi-component staphylococcal leucotoxins family

被引：135

作者：

Gravet, A ^{[1
]}

Colin, DA ^{[1
]}

Keller, D ^{[1
]}

Giradot, R ^{[1
]}

Monteil, H ^{[1
]}

Prévost, G ^{[1
]}

机构：

[1] Univ Louis Pasteur Hop, Fac Med, Inst Bacteriol, UPRES EA 1318, F-67000 Strasbourg, France

来源：

FEBS LETTERS | 1998年 / 436卷 / 02期

关键词：

characterization; bi-component leucotoxin; LukE+LukD; Ca2+/ethidium penetration; dermonecrosis; Staphylococcus aureus;

D O I：

10.1016/S0014-5793(98)01130-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A new member of the staphylococcal bi-component leucotoxins family, LukE (32 kDa) and LukD (34.3 kDa) has been characterized from Staphylococcus aureus strain Newman, LukE was 58-68% identical with the class S proteins, whereas LukD was 71-77% identical with the class E proteins of the family. A partial immunoreactivity with the various affinity-purified antibodies specific for the other proteins was observed, Immunoprecipitation assay and gene probing confirmed a 30% frequency among human clinical isolates, differing from the distribution of the other known leucotoxins (P < 0.005). LukE+LukD was as effective as the Panton-Valentine leucocidin for inducing dermonecrosis when injected in the rabbit skin, but not hemolytic and poorly leucotoxic compared to other leucotoxins expressed by Staphylococcus aureus. (C) 1998 Federation of European Biochemical Societies.

引用

页码：202 / 208

页数：7

共 29 条

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