Development and evaluation of two nested PCR assays for the detection of Babesia bovis from cattle blood

被引：14

作者：

AbouLaila, Mahmoud ^{[1
]}

Yokoyama, Naoaki ^{[1
]}

Igarashi, Ikuo ^{[1
]}

机构：

[1] Obihiro Univ Agr & Vet Med, Res Unit Mol Diag, Natl Res Ctr Protozoan Dis, Obihiro, Hokkaido 0808555, Japan

来源：

VETERINARY PARASITOLOGY | 2010年 / 172卷 / 1-2期

关键词：

Nested PCR; B bovis; Detection; Blood; Cattle; POLYMERASE-CHAIN-REACTION; RHOPTRY-ASSOCIATED PROTEIN-1; LINKED-IMMUNOSORBENT-ASSAY; BIGEMINA; PARASITES; DNA; AMPLIFICATION; TRANSMISSION; PIROPLASMS; DIAGNOSIS;

D O I：

10.1016/j.vetpar.2010.04.011

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

We developed and evaluated two nested polymerase chain reaction (nPCR) assays for the diagnosis of Babesia bovis infection in cattle based on two membrane protein genes from B bows BBOV IV005650 (BV5650) and BBO_IV008970 (BV8970) The specificities and sensitivities of the tests were compared with B bovis Rhoptery associated protein 1 gene (RAP-1) nPCR. The specificity of the tests was 100% for B bovis DNA The sensitivities of nPCR to B bows from the in vitro cultured parasites were as low as 10(-8)% 10(-6)% and 10(-7)% parasitemia for BV5650 BV8970 and RAP-1 nPCR, respectively The nPCR detected as little as 1 fg genomic DNA per test for BV5650 and 100 fg per test for both BV8970 and RAP-1 genes For field applications the sensitivity was evaluated to a total of 165 field samples from Ghana Mongolia Brazil and Japan The nPCR assay of BV5650 was the most sensitive for the detection of B bovis from the field samples The BV5650 nPCR assay provides a good diagnostic tool for laboratory diagnostic assessment of B bovis infection in cattle worldwide (C) 2010 Elsevier B V All rights reserved

引用

页码：65 / 70

页数：6

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