Detection of protein-protein interactions in vivo based on protein splicing

被引：39

作者：

Ozawa, T ^{[1
]}

Umezawa, Y

机构：

[1] Univ Tokyo, Sch Sci, Dept Chem, Bunkyo Ku, Tokyo 1130033, Japan

[2] Japan Sci & Technol Corp, Tokyo, Japan

来源：

CURRENT OPINION IN CHEMICAL BIOLOGY | 2001年 / 5卷 / 05期

基金：

日本科学技术振兴机构;

关键词：

D O I：

10.1016/S1367-5931(00)00244-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In mammalian cells, protein-protein interactions constitute essential regulatory steps that modulate the activity of signaling pathways. In recent years, several approaches towards understanding the interactions have been developed. We describe herein a new method for detecting protein-protein interactions in vivo based on protein splicing and highlight some potential applications of this technique.

引用

页码：578 / 583

页数：6

共 36 条

[1] Improved efficiency Sos recruitment system: expression of the mammalian GAP reduces isolation of Ras GTPase false positives [J].

Aronheim, A .

NUCLEIC ACIDS RESEARCH, 1997, 25 (16) :3373-3374

[2] Isolation of an AP-1 repressor by a novel method for detecting protein-protein interactions [J].

Aronheim, A ;

Zandi, E ;

Hennemann, H ;

Elledge, SJ ;

Karin, M .

MOLECULAR AND CELLULAR BIOLOGY, 1997, 17 (06) :3094-3102

[3] Protein recruitment systems for the analysis of protein-protein interactions [J].

Aronheim, A .

BIOCHEMICAL PHARMACOLOGY, 2000, 60 (08) :1009-1013

[4] Epidermal growth factor receptor dimerization monitored in live cells [J].

Blakely, BT ;

Rossi, FMV ;

Tillotson, B ;

Palmer, M ;

Estelles, A ;

Blau, HM .

NATURE BIOTECHNOLOGY, 2000, 18 (02) :218-222

[5] The Ras recruitment system, a novel approach to the study of protein-protein interactions [J].

Broder, YC ;

Katz, S ;

Aronheim, A .

CURRENT BIOLOGY, 1998, 8 (20) :1121-1124

[6] Biosynthesis of a head-to-tail cyclized protein with improved biological activity [J].

Camarero, JA ;

Muir, TW .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1999, 121 (23) :5597-5598

[7] THE 2-HYBRID SYSTEM - A METHOD TO IDENTIFY AND CLONE GENES FOR PROTEINS THAT INTERACT WITH A PROTEIN OF INTEREST [J].

CHIEN, CT ;

BARTEL, PL ;

STERNGLANZ, R ;

FIELDS, S .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (21) :9578-9582

[8] Single-column purification of free recombinant proteins using a self-cleavable affinity tag derived from a protein splicing element [J].

Chong, SR ;

Mersha, FB ;

Comb, DG ;

Scott, ME ;

Landry, D ;

Vence, LM ;

Perler, FB ;

Benner, J ;

Kucera, RB ;

Hirvonen, CA ;

Pelletier, JJ ;

Paulus, H ;

Xu, MQ .

GENE, 1997, 192 (02) :271-281

[9] Crystal structure of firefly luciferase throws light on a superfamily of adenylate-forming enzymes [J].

Conti, E ;

Franks, NP ;

Brick, P .

STRUCTURE, 1996, 4 (03) :287-298

[10] Insertion of a synthetic peptide into a recombinant protein framework: A protein biosensor [J].

Cotton, GJ ;

Ayers, B ;

Xu, R ;

Muir, TW .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1999, 121 (05) :1100-1101

← 1 2 3 4 →