A new facile method for spin-labeling of oligonucleotides

被引：24

作者：

Strube, T ^{[1
]}

Schiemann, O ^{[1
]}

MacMillan, F ^{[1
]}

Prisner, T ^{[1
]}

Engels, JW ^{[1
]}

机构：

[1] Univ Frankfurt, Inst Organ Chem, D-60439 Frankfurt, Germany

来源：

NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS | 2001年 / 20卷 / 4-7期

关键词：

D O I：

10.1081/NCN-100002534

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A new facile method for spin-labeling suitable for DNA and RNA oligonucleotides is presented. The nitroxide 3-ethenyl-2,2,5,5-tetramethyl-pyrrolin-1-yloxy was directly introduced during automated solid-phase synthesis by a Pd(0) cross coupling reaction. The main advantages of this procedure are the small amount of spin-label needed for the derivatisation of the oligonucleotide and the high coupling efficiency on the solid phase.

引用

页码：1271 / 1274

页数：4

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