DNA triple helix stabilisation by a naphthylquinoline dimer

被引：22

作者：

Keppler, M

Zegrocka, O

Strekowski, L

Fox, KR

机构：

[1] Univ Southampton, Sch Biol Sci, Div Biochem & Mol Biol, Southampton SO16 7PX, Hants, England

[2] Georgia State Univ, Dept Chem, Atlanta, GA 30303 USA

来源：

FEBS LETTERS | 1999年 / 447卷 / 2-3期

关键词：

tripler ligand; footprinting; DNA triplex; bis-intercalator; naphthylquinoline;

D O I：

10.1016/S0014-5793(99)00284-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have used DNase I footprinting to examine the effect of a novel naphthylquinoline dimer, designed as a triplex-specific bis-intercalator, on the stability of intermolecular DNA triplexes. We find that this compound efficiently promotes tripler formation between the 9-mer oligonucleotide 5'-TTTTTTCTT and its oligopurine duplex target at concentrations as low as 0.1 mu M, enhancing the tripler stability by at least 1000-fold. This compound, which is the first reported example of a tripler bis-intercalator, is about 30 times more potent than the simple monofunctional ligand. (C) 1999 Federation of European Biochemical Societies.

引用

页码：223 / 226

页数：4

共 34 条

[1] The contribution of cytosine protonation to the stability of parallel DNA triple helices [J].