Improved Photon Yield from a Green Dye with a Reducing and Oxidizing System

被引：17

作者：

Le Gall, Antoine ^{[1
,2
]}

Dulin, David ^{[1
,2
]}

Clavier, Gilles ^{[3
]}

Meallet-Renault, Rachel ^{[3
]}

Bouyer, Philippe ^{[1
,2
]}

Perronet, Karen ^{[1
,2
]}

Westbrook, Nathalie ^{[1
,2
]}

机构：

[1] CNRS, Inst Opt, Lab Charles Fabry, F-91127 Palaiseau, France

[2] Univ Paris 11, F-91127 Palaiseau, France

[3] UniverSud, CNRS, ENS Cachan, PPSM, F-94230 Cachan, France

来源：

CHEMPHYSCHEM | 2011年 / 12卷 / 09期

关键词：

biophysics; electron transfer; fluorescence; photophysics; single-molecule studies; BODIPY-FL; FLUORESCENCE; ELECTRON; MICROSCOPY; LIGHT;

D O I：

10.1002/cphc.201100085

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

An optimized chemistry turns a small green dye into a useful label for single-molecule experiments where steric hindrance is an issue. Thanks to a reducing and oxidizing system combined to oxygen depletion, a single Bodipy (boron-dipyrromethene) FL fluorophore emits, on average, 20 times more photons around 510 nm (see picture) and its lifetime before photobleaching is increased by the same amount, reaching several seconds. Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

引用

页码：1657 / 1660

页数：4

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