Reduced photobleaching of BODIPY-FL

被引：11

作者：

Dulin, David ^{[1
]}

Le Gall, Antoine ^{[1
]}

Perronet, Karen ^{[1
]}

Soler, Nicolas ^{[2
]}

Fourmy, Dominique ^{[2
]}

Yoshizawa, Satoko ^{[2
]}

Bouyer, Philippe ^{[1
]}

Westbrook, Nathalie ^{[1
]}

机构：

[1] CNRS, Inst Opt, Lab Charles Fabry, Campus Polytech,2 Ave Fresnel, F-91127 Palaiseau, France

[2] CNRS, ICSN, Lab Chim Biol Struct, F-91190 Gif Sur Yvette, France

来源：

PROCEEDINGS OF THE TENTH INTERNATIONAL MEETING ON HOLE BURNING, SINGLE MOLECULE AND RELATED SPECTROSCOPIES: SCIENCE AND APPLICATIONS-HBSM 2009 | 2010年 / 3卷 / 04期

关键词：

Type your keywords here; separated by semicolons; BODIPY-FL; photobleaching : single molecule fluorescence microscopy; oxygen scavenger; redox; FLUORESCENCE; BLINKING; SYSTEM;

D O I：

10.1016/j.phpro.2010.01.222

中图分类号：

TH7 [仪器、仪表];

学科分类号：

0804 ; 080401 ; 081102 ;

摘要：

We present a study of the photophysical properties of the BODIPY-FL fluorescent dye at the single molecule scale using total internal reflexion fluorescence microscopy. We characterized the saturation intensity and the maximum number of fluorescence photons for single BODIPY-FL in a usual buffer. We then studied the influence of the buffer depletion in oxygen and the addition of redox agents on the brightness and the lifetime before photobleaching of the BODIPY-FL. The great improvement of these two factors in such a medium allows the use of BODIPY-FL in single-molecule biophysics experiments. (C) 2010 Elsevier B. V. All rights reserved

引用

页码：1563 / 1567

页数：5

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