Mass production and dynamic imaging of fluorescent nanodiamonds

被引:644
作者
Chang, Yi-Ren [1 ]
Lee, Hsu-Yang [1 ]
Chen, Kowa [1 ]
Chang, Chun-Chieh [2 ]
Tsai, Dung-Sheng [1 ]
Fu, Chi-Cheng [1 ]
Lim, Tsong-Shin [1 ]
Tzeng, Yan-Kai [1 ]
Fang, Chia-Yi [1 ,3 ]
Han, Chau-Chung [1 ]
Chang, Huan-Cheng [1 ,3 ]
Fann, Wunshain [1 ,2 ,4 ]
机构
[1] Acad Sinica, Inst Atom & Mol Sci, Taipei 106, Taiwan
[2] Natl Taiwan Univ, Dept Phys, Taipei 106, Taiwan
[3] Natl Taiwan Normal Univ, Dept Chem, Taipei 106, Taiwan
[4] Natl Taiwan Univ, Inst Polymer Sci & Engn, Taipei 106, Taiwan
关键词
D O I
10.1038/nnano.2008.99
中图分类号
TB3 [工程材料学];
学科分类号
0805 ; 080502 ;
摘要
Fluorescent nanodiamond is a new nanomaterial that possesses several useful properties, including good biocompatibility(1), excellent photostability(1,2) and facile surface functionalizability(2,3). Moreover, when excited by a laser, defect centres within the nanodiamond emit photons that are capable of penetrating tissue, making them well suited for biological imaging applications(1,2,4). Here, we show that bright fluorescent nanodiamonds can be produced in large quantities by irradiating synthetic diamond nanocrystallites with helium ions. The fluorescence is sufficiently bright and stable to allow three-dimensional tracking of a single particle within the cell by means of either one- or two-photon-excited fluorescence microscopy. The excellent photophysical characteristics are maintained for particles as small as 25 nm, suggesting that fluorescent nanodiamond is an ideal probe for long-term tracking and imaging in vivo, with good temporal and spatial resolution.
引用
收藏
页码:284 / 288
页数:5
相关论文
共 31 条
[1]   Bacteria-mediated delivery of nanoparticles and cargo into cells [J].
Akin, Demir ;
Sturgis, Jennifer ;
Ragheb, Kathy ;
Sherman, Debby ;
Burkholder, Kristin ;
Robinson, J. Paul ;
Bhunia, Arun K. ;
Mohammed, Sulma ;
Bashir, Rashid .
NATURE NANOTECHNOLOGY, 2007, 2 (07) :441-449
[2]   Guiding a confocal microscope by single fluorescent nanoparticles [J].
Cang, Hu ;
Xu, C. Shan ;
Montiel, Daniel ;
Yang, Haw .
OPTICS LETTERS, 2007, 32 (18) :2729-2731
[3]   One at a time, live tracking of NGF axonal transport using quantum dots [J].
Cui, Bianxiao ;
Wu, Chengbiao ;
Chen, Liang ;
Ramirez, Alfredo ;
Bearer, Elaine L. ;
Li, Wei-Ping ;
Mobley, William C. ;
Chu, Steven .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2007, 104 (34) :13666-13671
[4]   OPTICAL STUDIES OF 1.945 EV VIBRONIC BAND IN DIAMOND [J].
DAVIES, G ;
HAMER, MF .
PROCEEDINGS OF THE ROYAL SOCIETY OF LONDON SERIES A-MATHEMATICAL AND PHYSICAL SCIENCES, 1976, 348 (1653) :285-298
[5]   Photo-induced creation of nitrogen-related color centers in diamond nanocrystals under femtosecond illumination [J].
Dumeige, Y ;
Treussart, F ;
Alléaume, R ;
Gacoin, T ;
Roch, JF ;
Grangier, P .
JOURNAL OF LUMINESCENCE, 2004, 109 (02) :61-67
[6]   Characterization and application of single fluorescent nanodiamonds as cellular biomarkers [J].
Fu, Chi-Cheng ;
Lee, Hsu-Yang ;
Chen, Kowa ;
Lim, Tsong-Shin ;
Wu, Hsiao-Yun ;
Lin, Po-Keng ;
Wei, Pei-Kuen ;
Tsao, Pei-Hsi ;
Chang, Huan-Cheng ;
Fann, Wunshain .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2007, 104 (03) :727-732
[7]   A superhighway to virus infection [J].
Greber, UF ;
Way, M .
CELL, 2006, 124 (04) :741-754
[8]   Scanning confocal optical microscopy and magnetic resonance on single defect centers [J].
Gruber, A ;
Drabenstedt, A ;
Tietz, C ;
Fleury, L ;
Wrachtrup, J ;
vonBorczyskowski, C .
SCIENCE, 1997, 276 (5321) :2012-2014
[9]  
Gu M., 1996, PRINCIPLES 3 DIMENSI
[10]   Deep tissue two-photon microscopy [J].
Helmchen, F ;
Denk, W .
NATURE METHODS, 2005, 2 (12) :932-940