Bacteria-mediated delivery of nanoparticles and cargo into cells

被引:273
作者
Akin, Demir
Sturgis, Jennifer
Ragheb, Kathy
Sherman, Debby
Burkholder, Kristin
Robinson, J. Paul
Bhunia, Arun K.
Mohammed, Sulma
Bashir, Rashid
机构
[1] Purdue Univ, Birck Nanotechnol Ctr, W Lafayette, IN 47907 USA
[2] Purdue Univ, Bindley Biosci Ctr, W Lafayette, IN 47907 USA
[3] Purdue Univ, Weldon Sch Biomed Engn, W Lafayette, IN 47907 USA
[4] Purdue Univ, Dept Basic Med Sci, W Lafayette, IN 47907 USA
[5] Purdue Univ, Dept Biol, W Lafayette, IN 47907 USA
[6] Purdue Univ, Dept Food Sci, Mol Food Microbiol Lab, W Lafayette, IN 47907 USA
[7] Dept Comparat Pathobiol, W Lafayette, IN 47907 USA
[8] Purdue Univ, Sch Elect & Comp Engn, W Lafayette, IN 47907 USA
关键词
LISTERIA-MONOCYTOGENES; IN-VIVO; MAMMALIAN-CELLS; DNA; PROTEIN; EXPRESSION; CANCER; TUMORS; BACTOFECTION; VACCINATION;
D O I
10.1038/nnano.2007.149
中图分类号
TB3 [工程材料学];
学科分类号
0805 ; 080502 ;
摘要
Nanoparticles and bacteria can be used, independently, to deliver genes and proteins into mammalian cells for monitoring or altering gene expression and protein production. Here, we show the simultaneous use of nanoparticles and bacteria to deliver DNA-based model drug molecules in vivo and in vitro. In our approach, cargo (in this case, a fluorescent or a bioluminescent gene) is loaded onto the nanoparticles, which are carried on the bacteria surface. When incubated with cells, the cargo- carrying bacteria ('microbots') were internalized by the cells, and the genes released from the nanoparticles were expressed in the cells. Mice injected with microbots also successfully expressed the genes as seen by the luminescence in different organs. This new approach may be used to deliver different types of cargo into live animals and a variety of cells in culture without the need for complicated genetic manipulations.
引用
收藏
页码:441 / 449
页数:9
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