Specific identification of Lactobacillus helveticus by PCR with pepC, pepN and htrA targeted primers

被引：31

作者：

Fortina, MG ^{[1
]}

Ricci, G ^{[1
]}

Mora, D ^{[1
]}

Parini, C ^{[1
]}

Manachini, PL ^{[1
]}

机构：

[1] Univ Milan, Dipartimento Sci & Tecnol Alimentari & Microbiol, Sez Microbiol Ind, I-20133 Milan, Italy

来源：

FEMS MICROBIOLOGY LETTERS | 2001年 / 198卷 / 01期

关键词：

Lactobacillus helveticus; bacterial identification; aminopeptidase and trypsin-like serine protease gene; polymerase chain reaction; species-specific probe;

D O I：

10.1111/j.1574-6968.2001.tb10623.x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Specific regions in three genes coding for aminopeptidases C and N, and a trypsin-like serine protease were selected as species-specific primer sequences for rapid and reliable identification of Lactobacillus helveticus strains. The PCR procedures carried out gave specific 524-, 726- and 918-bp amplificates, with DNA isolated from L. helveticus. No PCR product was generated for closely related bacteria. The amplification products were also screened for their species specificity in dot blot hybridization with representatives of the most closely related genera and species and a number of other bacterial species. (C) 2001 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：85 / 89

页数：5

共 15 条

[1] DELOSREYESGAVILAN CG, 1992, APPL ENVIRON MICROB, V58, P3429
[2] Fortina MG, 1998, J APPL MICROBIOL, V84, P72, DOI 10.1046/j.1365-2672.1997.00312.x
[3] Slow milk coagulating variants of Lactobacillus helveticus
Fortina, MG
Rossi, P
Mora, D
Parini, C
Neviani, E
[J]. FOLIA MICROBIOLOGICA, 1996, 41 (01) : 33 - 38
[4] FORTINA MG, 1990, BIOTECHNOL LETT, V12, P765, DOI 10.1007/BF01024737
[5] Genotypic heterogeneity among Lactobacillus helveticus strains isolated from natural cheese starters
Giraffa, G
De Vecchi, P
Rossi, P
Nicastro, G
Fortina, MG
[J]. JOURNAL OF APPLIED MICROBIOLOGY, 1998, 85 (03) : 411 - 416
[6] Different Lactobacillus helveticus strain populations dominate during Grana Padano cheesemaking
Giraffa, G
Neviani, E
[J]. FOOD MICROBIOLOGY, 1999, 16 (02) : 205 - 210
[7] Kandler O., 1986, BERGEYS MANUAL SYSTE, V2, P1208
[8] Development of molecular RAPD marker for the identification of Pediococcus acidilactici strains
Mora, D
Parini, C
Fortina, MG
Manachini, PL
[J]. SYSTEMATIC AND APPLIED MICROBIOLOGY, 2000, 23 (03) : 400 - 408
[9] Genetic (RAPD-PCR) and technological diversities among wild Lactobacillus helveticus strains
Quiberoni, A
Tailliez, P
Quénée, P
Suárez, V
Reinheimer, J
[J]. JOURNAL OF APPLIED MICROBIOLOGY, 1998, 85 (03) : 591 - 596
[10] PHENOTYPIC VARIABILITY AMONG CELLS OF LACTOBACILLUS-HELVETICUS ATCC-15807
REINHEIMER, JA
MORELLI, L
BOTTAZZI, V
SUAREZ, V
[J]. INTERNATIONAL DAIRY JOURNAL, 1995, 5 (01) : 97 - 103

← 1 2 →