Direct and simultaneous determination of representative byproducts in a lignocellulosic hydrolysate of corn stover via gas chromatography-mass spectrometry with a Deans switch

被引:12
作者
Zheng, Rongping [1 ]
Zhang, Hongman [1 ]
Zhao, Jing [1 ]
Lei, Mingliu [1 ]
Huang, He [2 ]
机构
[1] Nanjing Univ Technol, Coll Sci, Nanjing 210009, Peoples R China
[2] Nanjing Univ Technol, State Key Lab Mat Oriented Chem Engn, Coll Biotechnol & Pharmaceut Engn, Nanjing 210009, Peoples R China
基金
中国国家自然科学基金;
关键词
GC-MS; Deans switch; Byproduct; Lignocellulosic hydrolysate; VOLATILE ORGANIC-ACIDS; DEGRADATION-PRODUCTS; FERMENTATION INHIBITORS; PHASE MICROEXTRACTION; LIQUID-CHROMATOGRAPHY; PHENOLIC-COMPOUNDS; AROMATIC MONOMERS; PRETREATMENT; HPLC; ETHANOL;
D O I
10.1016/j.chroma.2011.05.099
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Pretreatment is one of the most important steps in producing fuel ethanol from lignocellulosic biomass. Simple, fast and accurate quantification of byproducts in lignocellulosic hydrolysates is critical to optimize the pretreatment procedures, but still a challenge. In this paper, a new GC-MS (SIM) method based on a Deans switch has been developed for the determination of byproducts in a corn stover hydrolysate. The Deans switch was incorporated into a hardware system that facilitated the direct aqueous injection (DAI) on GC-MS system. Simultaneous chromatographic separation and quantification of 18 byproducts including four aliphatic acids, five furan derivatives, four phenolic compounds and five others were achieved within 45 min. The detection limits of the presented method for various byproducts were in the range of 0.007-0.832 mg/L. The within-day and between-day precisions of the method were less than 6.0% (RSD, n = 6). The accuracy of the method was confirmed with recoveries of 86-128%. A lignocellulosic hydrolysate sample of corn stover was successfully analyzed using this method, with aliphatic acids and furan derivatives accounting for 89.15% of the selected total byproducts. (C) 2011 Elsevier B.V. All rights reserved.
引用
收藏
页码:5319 / 5327
页数:9
相关论文
共 44 条
[11]   Pressure-adjusted continual flow heart-cutting for the high throughput determination of amphetamine-type stimulant drugs in whole blood by fast multidimensional gas chromatography-mass spectrometry [J].
Gunnar, Teemu ;
Engblom, Charlotta ;
Ariniemi, Kari .
JOURNAL OF CHROMATOGRAPHY A, 2007, 1166 (1-2) :171-180
[12]   Mass Spectral Analyses of Corn Stover Prehydrolysates To Assess Conditioning Processes [J].
Helm, Richard F. ;
Jervis, Judith ;
Ray, W. Keith ;
Willoughby, Nicholas ;
Irvin, Benjamin ;
Hastie, Jessica ;
Schell, Daniel J. ;
Nagle, Nick .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2010, 58 (24) :12642-12649
[13]   Pretreatments to enhance the digestibility of lignocellulosic biomass [J].
Hendriks, A. T. W. M. ;
Zeeman, G. .
BIORESOURCE TECHNOLOGY, 2009, 100 (01) :10-18
[14]   Analysis of methyl tert.-butyl ether and its degradation products by direct aqueous injection onto gas chromatography with mass spectrometry or flame ionization detection systems [J].
Hong, S ;
Duttweiler, CM ;
Lemley, AT .
JOURNAL OF CHROMATOGRAPHY A, 1999, 857 (1-2) :205-216
[15]   Rapid quantification of major reaction products formed during thermochemical pretreatment of lignocellulosic biomass using GC-MS [J].
Humpula, James F. ;
Chundawat, Shishir P. S. ;
Vismeh, Ramin ;
Jones, A. Daniel ;
Balan, Venkatesh ;
Dale, Bruce E. .
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2011, 879 (13-14) :1018-1022
[16]   Two-dimensional gas chromatography/electron-impact mass spectrometry with cryofocusing for simultaneous quantification of MDMA, MDA, HMMA, HMA, and MDEA in human plasma [J].
Kolbrich, Erin A. ;
Lowe, Ross H. ;
Huestis, Marilyn A. .
CLINICAL CHEMISTRY, 2008, 54 (02) :379-387
[17]   The generation of fermentation inhibitors during dilute acid hydrolysis of softwood [J].
Larsson, S ;
Palmqvist, E ;
Hahn-Hägerdal, B ;
Tengborg, C ;
Stenberg, K ;
Zacchi, G ;
Nilvebrant, NO .
ENZYME AND MICROBIAL TECHNOLOGY, 1999, 24 (3-4) :151-159
[18]   Simultaneous HPLC determination of sugars, organic acids and ethanol in sourdough process [J].
Lefebvre, D ;
Gabriel, V ;
Vayssier, Y ;
Fontagné-Faucher, C .
LEBENSMITTEL-WISSENSCHAFT UND-TECHNOLOGIE-FOOD SCIENCE AND TECHNOLOGY, 2002, 35 (05) :407-414
[19]   Genomic adaptation of ethanologenic yeast to biomass conversion inhibitors [J].
Liu, Z. Lewis .
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2006, 73 (01) :27-36
[20]   Determination of phenolic compounds from oxidation of lignin lake sediments by high-performance liquid chromatography [J].
Lobo, I ;
Mozeto, AA ;
Cass, QB .
CHROMATOGRAPHIA, 2000, 52 (11-12) :727-731