Molecular strain typing of Campylobacter jejuni by pulsed-field gel electrophoresis in a single day

被引：8

作者：

Michaud, S

Arbeit, RD

Gaudreau, C

机构：

[1] Vet Adm Med Ctr Jamaica Plain, Res Serv, Boston, MA 02130 USA

[2] Boston Univ, Sch Med, Dept Med, Boston, MA 02130 USA

[3] Ctr Hosp Univ Montreal, Hop St Luc, Dept Microbiol Med & Infectiol, Montreal, PQ H2X 3J4, Canada

来源：

CANADIAN JOURNAL OF MICROBIOLOGY | 2001年 / 47卷 / 07期

关键词：

pulsed-field gel electrophoresis; Campylobacter jejuni; molecular epidemiology;

D O I：

10.1139/cjm-47-7-667

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Rapid molecular strain typing is critical for effective outbreak investigation and implementation of infection control measures. Pulsed-field gel electrophoresis is a highly discriminatory technique for Campylobacter jejuni, but generally requires 3-5 days. We describe a simplified protocol for pulsed-field gel electrophoresis that provides high quality typing of C. jejuni isolates in a single day.

引用

页码：667 / 669

页数：3

共 12 条

[1]

*CDCP, 1999, STAND MOL SUBT ESCH

[2] Rapid pulsed-field gel electrophoresis protocol for typing of Escherichia coli O157:H7 and other gram-negative organisms in 1 day [J].