An improved in vitro technique for isolated microspore culture of barley

被引：60

作者：

Kasha, KJ ^{[1
]}

Simion, E ^{[1
]}

Oro, R ^{[1
]}

Yao, QA ^{[1
]}

Hu, TC ^{[1
]}

Carlson, AR ^{[1
]}

机构：

[1] Univ Guelph, Dept Plant Agr, Div Biotechnol, Guelph, ON N1G 2W1, Canada

来源：

EUPHYTICA | 2001年 / 120卷 / 03期

基金：

加拿大自然科学与工程研究理事会;

关键词：

barley; cell culture; haploid; induction; microspore; regeneration;

D O I：

10.1023/A:1017564100823

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

A detailed procedure for isolated microspore culture of barley is presented along with examples of response across genotypes. Over 30 genotypes, including winter and spring growth habit and 2-row and 6-row genotypes, have shown an essentially genotype independent response, averaging about 10,000 embryos per 5 cm petri culture plate. The regeneration frequency, checked on samples of 500 embryos per plate ranged from 36 to 97% with most genotypes being in the range of 70 to 90%. About 70 to 80% of the plants regenerated have been completely fertile doubled haploids, thus eliminating the need to double the chromosome number of plants. Many little details are critical to success of the microspore procedure and while it saves much time compared to anther culture, greater attention to details and cleanliness is essential.

引用

页码：379 / 385

页数：7