Measurement of the malondialdehyde-2′-deoxyguanosine adduct in human urine by immunoextraction and liquid chromatography/atmospheric pressure chemical ionization tandem mass spectrometry

The major adduct of malondialdehyde with guanine, M(1)G, was measured in human urine from nonsmoking healthy individuals. M(1)G is a mutagenic DNA lesion and a terminal product of lipid peroxidation in vivo that may be implicated in cancer related to lifestyle and diet. On the basis of a recently developed method for the quantification of M(1)G as an excreted deoxynucleoside using immuno-extraction purification, chemical NaBH4 reduction and liquid chromatography combined with atmospheric pressure chemical ionization tandem mass spectrometry, we demonstrate that the average 24 h excretion rate of M(1)G-dR is about 12+/-3.8 fmol kg(-1) (n = 5). Copyright (C) 2004 John Wiley Sons, Ltd.