Mixotrophic cultivation of Platymonas subcordiformis

The effects of glucose concentration and irradiance on mixotrophic cultivation of Platymonas subcordiformis were investigated in flask cultures. The optimal glucose concentration was 24 g L-1, and irradiance was 95 mu mol photon m(-2) s(-1). Based on the optimization of culture conditions and a modified SK(IA) medium, the maximum biomass was 3.68 g L-1 after 14 days, which was about 6 times that in autotrophic culture, and the specific growth rate reached 0.62 d(-1), twice that in autotrophic culture. Using the optimal conditions established in flasks, mixotrophic culture in a 5.0-L photobioreactor was achieved, and the yield after 8 days was 3.30 g L-1.