Immunolocalization of non-extractable (bound) residues of pesticides and industrial contaminants in plants and soil

被引：14

作者：

Dankwardt, A ^{[1
]}

Hock, B ^{[1
]}

机构：

[1] Tech Univ Munich, Dept Bot, D-85350 Freising Weihenstephan, Germany

来源：

CHEMOSPHERE | 2001年 / 45卷 / 4-5期

关键词：

immunoassay; antibodies; fluorescence labeling; atrazine; TNT;

D O I：

10.1016/S0045-6535(00)00603-2

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

The application of immunochemical. methods for the investigation of non-extractable (bound) residues is reviewed. Non-extractable residues may be presented to antibodies as antigenic determinants, which are exposed for instance in plant tissue and humic substances. Fluorescent probes as well as enzyme markers have been applied for the detection of bound residues. The application of antibodies labeled with fluorescein isothiocyanate (FITC) and phycoerythrin revealed the presence of atrazine in cryosections of atrazine-treated corn leaves and water plants. Atrazine could be localized by antibodies coupled to fluorescent markers in soil from corn fields but not in atrazine-free soil. Quantitative results were obtained by the application of enzyme immunoassays to the investigation of triazine and 2,4,6-trinitrotoluene (TNT) residues, bound to soil humic acids. Finally, the use of antibodies with different recognition patterns provides information on the ligation of non-extractable residues to the matrix. (C) 2001 Elsevier Science Ltd. All rights reserved.

引用

页码：523 / 533

页数：11

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