Chromosomal constraints in Gram-positive bacteria revealed by artificial inversions

被引：50

作者：

Campo, N ^{[1
]}

Dias, MJ ^{[1
]}

Daveran-Mingot, ML ^{[1
]}

Ritzenthaler, P ^{[1
]}

Le Bourgeois, P ^{[1
]}

机构：

[1] Univ Toulouse 3, CNRS, UMR5100, Lab Microbiol & Genet Mol, F-31062 Toulouse, France

来源：

MOLECULAR MICROBIOLOGY | 2004年 / 51卷 / 02期

关键词：

D O I：

10.1046/j.1365-2958.2003.03847.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We used artificial chromosome inversions to investigate the chromosomal constraints that preserve genome organization in the Gram-positive bacterium Lactococcus lactis. Large inversions, 80-1260 kb in length, disturbing the symmetry of the origin and terminus of the replication axis to various extents, were constructed using the site-specific Cre-loxP recombination system. These inversions were all mechanistically feasible and fell into various classes according to stability and effect on cell fitness. The L. lactis chromosome supports only to some extent unbalance in length of its replication arms. The location of detrimental inversions allowed identification of two constrained chromosomal regions: a large domain covering one fifth of the genome that encompasses the origin of replication (Ori domain), and a smaller domain located at the opposite of the chromosome (Ter domain).

引用

页码：511 / 522

页数：12

共 67 条

[1] STUDIES ON THE PROPERTIES OF P1 SITE-SPECIFIC RECOMBINATION - EVIDENCE FOR TOPOLOGICALLY UNLINKED PRODUCTS FOLLOWING RECOMBINATION [J].