Inter-kingdom conservation of mechanism of nonsense-mediated mRNA decay

被引：132

作者：

Kerenyi, Zoltan ^{[1
]}

Merai, Zsuzsanna ^{[1
]}

Hiripi, Laszlo ^{[1
]}

Benkovics, Anna ^{[1
]}

Gyula, Peter ^{[2
]}

Lacomme, Christophe ^{[3
,4
]}

Barta, Endre ^{[1
]}

Nagy, Ferenc ^{[2
]}

Silhavy, Daniel ^{[1
]}

机构：

[1] Agr Biotechnol Ctr, H-2100 Godollo, Hungary

[2] Hungarian Acad Sci, Biol Res Ctr, Inst Plant Biol, H-6701 Szeged, Hungary

[3] SCRI, Dundee, Scotland

[4] Univ Edinburgh, Inst Mol Plant Sci, Edinburgh, Midlothian, Scotland

来源：

EMBO JOURNAL | 2008年 / 27卷 / 11期

关键词：

EJC; NMD; SMG-7; UPF; VIGS;

D O I：

10.1038/emboj.2008.88

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Nonsense-mediated mRNA decay (NMD) is a quality control system that degrades mRNAs containing premature termination codons. Although NMD is well characterized in yeast and mammals, plant NMD is poorly understood. We have undertaken the functional dissection of NMD pathways in plants. Using an approach that allows rapid identification of plant NMD trans factors, we demonstrated that two plant NMD pathways coexist, one eliminates mRNAs with long 30UTRs, whereas a distinct pathway degrades mRNAs harbouring 30UTR-located introns. We showed that UPF1, UPF2 and SMG-7 are involved in both plant NMD pathways, whereas Mago and Y14 are required only for intron-based NMD. The molecular mechanism of long 30UTR-based plant NMD resembled yeast NMD, whereas the intron-based NMD was similar to mammalian NMD, suggesting that both pathways are evolutionarily conserved. Interestingly, the SMG-7 NMD component is targeted by NMD, suggesting that plant NMD is autoregulated. We propose that a complex, autoregulated NMD mechanism operated in stem eukaryotes, and that despite aspect of the mechanism being simplified in different lineages, feedback regulation was retained in all kingdoms.

引用

页码：1585 / 1595

页数：11

共 44 条

[31] Biochemical and cellular characterization of the plant ortholog of PYM, a protein that interacts with the exon junction complex core proteins Mago and Y14 [J].