Terminal phosphate labeled nucleotides: Synthesis, applications, and linker effect on incorporation by DNA polymerases

被引：29

作者：

Kumar, S ^{[1
]}

Sood, A ^{[1
]}

Wegener, J ^{[1
]}

Finn, PJ ^{[1
]}

Nampalli, S ^{[1
]}

Nelson, JR ^{[1
]}

Sekher, A ^{[1
]}

Mitsis, P ^{[1
]}

Macklin, J ^{[1
]}

Fuller, CW ^{[1
]}

机构：

[1] GE Healthcare, Piscataway, NJ 08855 USA

来源：

NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS | 2005年 / 24卷 / 5-7期

关键词：

terminal phosphate labeled nucleotides; linkers; dyes; DNA polymerase extension; homogenous assays;

D O I：

10.1081/NCN-200059823

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A number of terminal phosphate-labeled nucleotides with three or more phosphates and with varied length linkers attached between the terminal phosphate and the dye have been synthesized. These nucleotides have been tested as substrates for different DNA and RNA polymerases. We have also explored their utility in DNA sequencing, SNP analysis, nucleic acid amplification, quantitative PCR, and other biochemical assays.

引用

页码：401 / 408

页数：8

共 12 条

[1] gamma-phosphate-substituted 2'-deoxynucleoside 5'-triphosphates as substrates for DNA polymerases [J].