The slit receptor EVA-1 coactivates a SAX-3/robo-mediated guidance signal in C-elegans

被引：47

作者：

Fujisawa, Kazuko

Wrana, Jeffrey L.

Culotti, Joseph G.

机构：

[1] Mt Sinai Hosp, Samuel Lunenfeld Res Inst, Toronto, ON M5G 1X5, Canada

[2] Univ Toronto, Dept Mol & Med Genet, Toronto, ON M5S 1A8, Canada

来源：

SCIENCE | 2007年 / 317卷 / 5846期

关键词：

D O I：

10.1126/science.1144874

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 [理学]; 0710 [生物学]; 09 [农学];

摘要：

The SAX-3/roundabout (Robo) receptor has Shiga-like toxin 1 (SLT-1)/Slit-dependent and -independent functions in guiding cell and axon migrations. We identified enhancer of ventral-axon guidance defects of unc-40 mutants (EVA-1) as a Caenorhabditis elegans transmembrane receptor for SLT-1. EVA-1 has two predicted galactose-binding ectodomains, acts cell-autonomously for SLT-1/Slit-dependent axon migration functions of SAX-3/Robo, binds to SLT-1 and SAX-3, colocalizes with SAX-3 on cells, and provides cell specificity to the activation of SAX-3 signaling by SLT-1. Double mutants of eva-1 or slt-1 with sax-3 mutations suggest that SAX-3 can (when slt-1 or eva-1 function is reduced) inhibit a parallel-acting guidance mechanism, which involves UNC-40/deleted in colorectal cancer.

引用

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页码：1934 / 1938

页数：5

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