Differences in the expression of lipolytic-related genes in rat white adipose tissues

被引:25
作者
Bertile, F [1 ]
Criscuolo, F [1 ]
Oudart, H [1 ]
Le Maho, Y [1 ]
Raclot, T [1 ]
机构
[1] Univ Strasbourg, Ctr Ecol & Physiol Energet, UPR 9010, CNRS, F-67087 Strasbourg 2, France
关键词
lipolysis; body fat distribution; gene expression; fasting/refeeding; time-course changes;
D O I
10.1016/S0006-291X(03)01196-3
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We have investigated in vivo whether the gene expression of the beta3-adrenergic receptor (beta3-AR), perilipin A, hormone-sensitive lipase (HSL), and adipocyte lipid-binding protein (ALBP/aP2) is regulated in a site-specific manner. To induce lipolysis and discriminate between short- and long-term modifications, rats were submitted to an experimental fast for one or five days followed or not by refeeding. The mRNA encoding beta3-AR in retroperitoneal adipose tissue (RP) was significantly increased by one and five days of fasting (4-fold) and then lowered by one day of refeeding (2-fold) compared to fed rats. The reverse trend was observed for perilipin A expression in one day fasted rats. HSL mRNA concentrations were significantly induced (2.2-fold) by five days of fasting relative to fed animals and remained high after refeeding. ALBP/aP2, peroxisome proliferator-activated receptor gamma, and CAAT/enhancer binding protein alpha mRNA levels were essentially unaffected by dietary manipulations. Fasting and/or refeeding were similarly ineffective at regulating gene expression in SC. These data provide a molecular basis for regional differences at different steps of the lipolytic process. (C) 2003 Elsevier Inc. All rights reserved.
引用
收藏
页码:540 / 546
页数:7
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