Improved hydrogen photoproduction regulated by carbonylcyanide m-chlorophenylhrazone from marine green alga Platymonas subcordiformis grown in CO2-supplemented air bubble column bioreactor

被引:33
作者
Guo, Zhen [1 ,2 ]
Chen, Zhaoan [1 ]
Zhang, Wei [1 ,3 ]
Yu, Xingju [1 ]
Jin, Meifang [1 ]
机构
[1] Chinese Acad Sci, Dalian Inst Chem Phys, Marine Bioprod Engn Grp, Dalian 116023, Peoples R China
[2] Grad Sch Chinese Acad Sci, Beijing 100039, Peoples R China
[3] Flinders Univ S Australia, Sch Med, Dept Med Biotechnol, Bedford Pk, SA 5042, Australia
关键词
bioreactor; carbon dioxide; carbonylcyanide m-chlorophenylhydrazone (CCCP); hydrogen; Platymonas subcordiformis;
D O I
10.1007/s10529-008-9637-1
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
To develop an integrated process of CO2- fixation and H-2 photoproduction by marine green microalga Platymonas subcordiformis, the impact of algal cells grown in CO2- supplemented air bubble column bioreactor was investigated on H-2 photoproduction regulated by carbonylcyanide m- chlorophenylhrazone. Highest cell growth ( 3.85 9 10(6) cells ml(-1)), starch content ( 0.25 +/- 0.08 mg per 10(6) cells) and hydrogen production ( 50 +/- 3 ml1(-1)) were achieved at 3% CO2- supplemented culture, which are respectively 1.4, 2.1, 1.5- fold of the air-supplemented culture. Improved H-2 production correlated well with the increase in starch accumulation. In this process, the algal cells have been recycled for stable H-2 production of 40 - 50 ml l(-1) over five cycles.
引用
收藏
页码:877 / 883
页数:7
相关论文
共 17 条
[1]   The relationship between the photosystem 2 activity and hydrogen production in sulfur deprived Chlamydomonas reinhardtii cells [J].
Antal T.K. ;
Krendeleva T.E. ;
Laurinavichene T.V. ;
Makarova V.V. ;
Tsygankov A.A. ;
Seibert M. ;
Rubin A.B. .
Doklady Biochemistry and Biophysics, 2001, 381 (1-6) :371-374
[2]   Fermentative and photochemical production of hydrogen in algae [J].
Gaffron, H ;
Rubin, J .
JOURNAL OF GENERAL PHYSIOLOGY, 1942, 26 (02) :219-240
[3]   Microalgae:: a green source of renewable H2 [J].
Ghirardi, ML ;
Zhang, JP ;
Lee, JW ;
Flynn, T ;
Seibert, M ;
Greenbaum, E ;
Melis, A .
TRENDS IN BIOTECHNOLOGY, 2000, 18 (12) :506-511
[4]   Significant enhancement of photobiological H2 evolution by carbonylcyanide m-chlorophenylhydrazone in the marine green alga Platymonas subcordiformis [J].
Guan, YF ;
Zhang, W ;
Deng, MC ;
Jin, MF ;
Yu, XJ .
BIOTECHNOLOGY LETTERS, 2004, 26 (13) :1031-1035
[5]   Two-stage photo-biological production of hydrogen by marine green alga Platymonas subcordiformis [J].
Guan, YF ;
Deng, MC ;
Yu, XJ ;
Zhang, W .
BIOCHEMICAL ENGINEERING JOURNAL, 2004, 19 (01) :69-73
[6]   Hydrogenases in green algae: do they save the algae's life and solve our energy problems? [J].
Happe, T ;
Hemschemeier, A ;
Winkler, M ;
Kaminski, A .
TRENDS IN PLANT SCIENCE, 2002, 7 (06) :246-250
[7]   The exceptional photofermentative hydrogen metabolism of the green alga Chlamydomonas reinhardtii [J].
Hemschemeier, A ;
Happe, T .
BIOCHEMICAL SOCIETY TRANSACTIONS, 2005, 33 :39-41
[8]   Effect of extremely high-CO2 stress on energy distribution between photosystem I and photosystem II in a 'high-CO2' tolerant green alga, Chlorococcum littorale and the intolerant green alga Stichococcus bacillaris [J].
Iwasaki, I ;
Hu, Q ;
Kurano, N ;
Miyachi, S .
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, 1998, 44 (03) :184-190
[9]   FERMENTATIVE METABOLISM OF HYDROGEN-EVOLVING CHLAMYDOMONAS-MOEWUSII [J].
KLEIN, U ;
BETZ, A .
PLANT PHYSIOLOGY, 1978, 61 (06) :953-956
[10]   Effects of extracellular pH on the metabolic pathways in sulfur-deprived, H2-producing Chlamydomonas reinhardtii cultures [J].
Kosourov, S ;
Seibert, M ;
Ghirardi, ML .
PLANT AND CELL PHYSIOLOGY, 2003, 44 (02) :146-155