A new signal sequence trap using alkaline phosphatase as a reporter

被引：23

作者：

Chen, HC ^{[1
]}

Leder, P ^{[1
]}

机构：

[1] Harvard Univ, Sch Med, Dept Genet, Howard Hughes Med Inst, Boston, MA 02115 USA

来源：

NUCLEIC ACIDS RESEARCH | 1999年 / 27卷 / 04期

关键词：

D O I：

10.1093/nar/27.4.1219

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Secreted and transmembrane proteins are critical to the cell-cell interactions governing normal development and carcinogenesis. To facilitate the identification of such molecules, we have developed a novel signal sequence trap that uses human placental alkaline phosphatase as a reporter. Libraries from mouse prostate and human prostatic carcinoma were constructed to test the PST (peptide signal trap) system, resulting in the identification of several secreted and transmembrane proteins.

引用

页码：1219 / 1222

页数：4

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