A new signal sequence trap using alkaline phosphatase as a reporter

被引：23

作者：

Chen, HC ^{[1
]}

Leder, P ^{[1
]}

机构：

[1] Harvard Univ, Sch Med, Dept Genet, Howard Hughes Med Inst, Boston, MA 02115 USA

来源：

NUCLEIC ACIDS RESEARCH | 1999年 / 27卷 / 04期

关键词：

D O I：

10.1093/nar/27.4.1219

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Secreted and transmembrane proteins are critical to the cell-cell interactions governing normal development and carcinogenesis. To facilitate the identification of such molecules, we have developed a novel signal sequence trap that uses human placental alkaline phosphatase as a reporter. Libraries from mouse prostate and human prostatic carcinoma were constructed to test the PST (peptide signal trap) system, resulting in the identification of several secreted and transmembrane proteins.

引用

页码：1219 / 1222

页数：4

共 16 条

[11] A BACILLUS-SUBTILIS SECRETION VECTOR SYSTEM DERIVED FROM THE B-SUBTILIS ALPHA-AMYLASE PROMOTER AND SIGNAL SEQUENCE REGION, AND SECRETION OF ESCHERICHIA-COLI BETA-LACTAMASE BY THE VECTOR SYSTEM [J].

OHMURA, K ;

SHIROZA, T ;

NAKAMURA, K ;

NAKAYAMA, A ;

YAMANE, K ;

YODA, K ;

YAMASAKI, M ;

TAMURA, G .

JOURNAL OF BIOCHEMISTRY, 1984, 95 (01) :87-93

[12] Identification of stromal cell products that interact with pre-B cells [J].

Oritani, K ;

Kincade, PW .

JOURNAL OF CELL BIOLOGY, 1996, 134 (03) :771-782

[13] CONSTRUCTION OF A NORMALIZED CDNA LIBRARY BY INTRODUCTION OF A SEMISOLID MESSENGER-RNA-CDNA HYBRIDIZATION SYSTEM [J].

SASAKI, YF ;

AYUSAWA, D ;

OISHI, M .

NUCLEIC ACIDS RESEARCH, 1994, 22 (06) :987-992

[14] SIGNAL SEQUENCE TRAP - A CLONING STRATEGY FOR SECRETED PROTEINS AND TYPE-I MEMBRANE-PROTEINS [J].

TASHIRO, K ;

TADA, H ;

HEILKER, R ;

SHIROZU, M ;

NAKANO, T ;

HONJO, T .

SCIENCE, 1993, 261 (5121) :600-603

[15]

Tashiro K, 1997, Methods Mol Biol, V69, P203

[16] A NEW METHOD FOR PREDICTING SIGNAL SEQUENCE CLEAVAGE SITES [J].

VONHEIJNE, G .

NUCLEIC ACIDS RESEARCH, 1986, 14 (11) :4683-4690

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