Autophagic machinery activated by dengue virus enhances virus replication

被引：317

作者：

Lee, Ying-Ray ^{[2
]}

Lei, Huan-Yao ^{[1
,2
]}

Liu, Ming-Tao ^{[3
]}

Wang, Jen-Ren ^{[4
]}

Chen, Shun-Hua ^{[1
]}

Jiang-Shieh, Ya-Fen ^{[5
]}

Lin, Yee-Shin ^{[1
]}

Yeh, Trai-Ming ^{[4
]}

Liu, Ching-Chang ^{[6
]}

Liu, Hsiao-Sheng ^{[1
]}

机构：

[1] Natl Cheng Kung Univ, Coll Med, Dept Microbiol & Immunol, Tainan 70101, Taiwan

[2] Natl Cheng Kung Univ, Coll Med, Inst Basic Med Sci, Tainan, Taiwan

[3] Tainan Hosp, Dept Hlth, Tainan, Taiwan

[4] Natl Cheng Kung Univ, Coll Med, Dept Med Lab Sci & Biotechnol, Tainan, Taiwan

[5] Natl Cheng Kung Univ, Coll Med, Dept Cell Biol & Anat, Tainan, Taiwan

[6] Natl Cheng Kung Univ, Coll Med, Dept Pediat, Tainan, Taiwan

来源：

VIROLOGY | 2008年 / 374卷 / 02期

关键词：

dengue virus; autophagy; autophagosome; viral replication;

D O I：

10.1016/j.virol.2008.02.016

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Autophagy is a cellular response against stresses which include the infection of viruses and bacteria. We unravel that Dengue virus-2 (DV2) can trigger autophagic process in various infected cell lines demonstrated by GFP-LC3 dot formation and increased LC3-II formation. Autophagosome formation was also observed under the transmission electron microscope. DV2-induced autophagy further enhances the titers of extracellular and intracellular viruses indicating that autophagy can promote viral replication in the infected cells. Moreover, our data show that ATG5 protein is required to execute DV2-induced autophagy. All together, we are the first to demonstrate that DV can activate autophagic machinery that is favorable for viral replication. (c) 2008 Elsevier Inc. All rights reserved.

引用

页码：240 / 248

页数：9

共 27 条

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