Quantitative imaging of nitrogen fixation by individual bacteria within animal cells

被引:197
作者
Lechene, Claude P.
Luyten, Yvette
McMahon, Gregory
Distel, Daniel L.
机构
[1] Harvard Univ, Sch Med, Natl Resource Imaging Mass Spectrometry, Cambridge, MA 02139 USA
[2] Brigham & Womens Hosp, Cambridge, MA 02139 USA
[3] Ctr Marine Genom Res, Ocean Genome Legacy, Ipswich, MA 01938 USA
关键词
D O I
10.1126/science.1145557
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Biological nitrogen fixation, the conversion of atmospheric nitrogen to ammonia for biosynthesis, is exclusively performed by a few bacteria and archaea. Despite the essential importance of biological nitrogen fixation, it has been impossible to quantify the incorporation of nitrogen by individual bacteria or to map the fate of fixed nitrogen in host cells. In this study, with multi-isotope imaging mass spectrometry we directly imaged and measured nitrogen fixation by individual bacteria within eukaryotic host cells and demonstrated that fixed nitrogen is used for host metabolism. This approach introduces a powerful way to study microbes and global nutrient cycles.
引用
收藏
页码:1563 / 1566
页数:4
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