A disposable immunomagnetic electrochemical sensor based on functionalised magnetic beads and carbon-based screen-printed electrodes (SPCEs) for the detection of polychlorinated biphenyls (PCBs)

被引:47
作者
Centi, S
Laschi, S
Fránek, M
Mascini, M
机构
[1] Univ Florence, Dipartimento Chim, Florence, Italy
[2] Vet Res Inst, Brno 62132, Czech Republic
关键词
screen-printed electrodes; immunoassay; polychlorinated biphenyls; electrochemical detection; magnetic beads;
D O I
10.1016/j.aca.2005.01.073
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
A disposable immunomagnetic electrochemical sensor involving magnetic particles and carbon-based screen-printed electrodes (SPCEs) was developed and applied for the detection of polychlorinated biphenyls (PCBs). The sensor was based on a direct competitive immunoassay scheme in which antibody-coated magnetic beads were used as solid phase; then SPCEs were employed as transducers for the evaluation of the extent of the immunochemical reaction using an alkaline phosphatase (AP) labelled tracer. The a-naphthyl phosphate was used as enzymatic substrate and the a-naphthol produced during the enzymatic reaction was detected using differential pulse voltammetry (DPV). A detection limit of 0.4 ng/mL for Aroclor 1248 PCB mixture was obtained. The performance of the sensor was successfully evaluated on marine sediment extracts and soil samples spiked with different Aroclor mixtures indicating that this new and sensitive technique offers great promise for decentralized environmental applications. (c) 2005 Elsevier B.V. All rights reserved.
引用
收藏
页码:205 / 212
页数:8
相关论文
共 20 条
[1]  
AHMED FE, 2003, TRENDS ANAL CHEM, V22, P3
[2]   Determination of polychlorinated biphenyls in ash using dimethylsulfoxide microwave assisted extraction followed by solid-phase microextraction [J].
Criado, MR ;
Pereiro, IR ;
Torrijos, RC .
TALANTA, 2004, 63 (03) :533-540
[3]  
Davies C., 1994, IMMUNOASSAY HDB, P83
[4]   Feasibility studies of simultaneous multianalyte amperometric immunoassay based on spatial resolution [J].
Ding, Y ;
Zhou, LP ;
Halsall, HB ;
Heineman, WR .
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 1999, 19 (1-2) :153-161
[5]   Relative performance of immunochemical (enzyme-linked immunosorbent assay) and gas chromatography-electron-capture detection techniques to quantify polychlorinated biphenyls in mussel tissues [J].
Fillmann, G ;
Galloway, TS ;
Sanger, RC ;
Depledge, MH ;
Readman, JW .
ANALYTICA CHIMICA ACTA, 2002, 461 (01) :75-84
[6]   Direct competitive immunoassays for the coplanar polychlorinated biphenyls [J].
Fránek, M ;
Deng, AP ;
Kolár, V ;
Socha, J .
ANALYTICA CHIMICA ACTA, 2001, 444 (01) :131-142
[7]   Enzyme-immunoassays for polychlorinated biphenyls: structural aspects of hapten-antibody binding [J].
Franek, M ;
Pouzar, V ;
Kolar, V .
ANALYTICA CHIMICA ACTA, 1997, 347 (1-2) :163-176
[8]   POLYCHLOROBIPHENYLS IN THE ENVIRONMENT - ANALYTICAL PROCEDURES AND DATA EVALUATION [J].
FUOCO, R ;
COLOMBINI, MP .
MICROCHEMICAL JOURNAL, 1995, 51 (1-2) :106-121
[9]   Polychlorinated dibenzo-p-dioxins, -dibenzofurans, and biphenyls in domestic animal food stuff and their fat [J].
Guruge, KS ;
Seike, N ;
Yamanaka, N ;
Miyazaki, S .
CHEMOSPHERE, 2005, 58 (07) :883-889
[10]   Quantitative ELISA of polychlorinated biphenyls in an oily soil matrix using supercritical fluid extraction [J].
Johnson, JC ;
Van Emon, JM ;
Clarke, AN ;
Wamsley, BN .
ANALYTICA CHIMICA ACTA, 2001, 428 (02) :191-199