Visualization by atomic force microscopy of tobacco mosaic virus movement protein-RNA complexes formed in vitro

被引:44
作者
Kiselyova, OI
Yaminsky, IV
Karger, EM
Frolova, OY
Dorokhov, YL
Atabekov, JG [1 ]
机构
[1] Moscow State Univ, Fac Phys, Moscow 119899, Russia
[2] Moscow State Univ, Fac Chem, Dept Virol, Moscow 119899, Russia
[3] Moscow State Univ, AN Belozersky Inst Physicochem Biol, Moscow 119899, Russia
关键词
D O I
10.1099/0022-1317-82-6-1503
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The structure of complexes formed in vitro by tobacco mosaic virus (TMV)-coded movement protein (MP) with TMV RNA and short (890 nt) synthetic RNA transcripts was visualized by atomic force microscopy on a mica surface. MP molecules were found to be distributed along the chain of RNA and the structure of MP-RNA complexes depended on the molar MP:RNA ratios at which the complexes were formed. A rise in the molar MP:TMV RNA ratio from 20:1 to 60-100: 1 resulted in an increase in the density of the MP packaging on TMV RNA and structural conversion of complexes from RNase-sensitive 'beads-on-a-string' into a 'thick string' form that was partly resistant to RNase. The 'thick string'-type RNase-resistant complexes were also produced by short synthetic RNA transcripts at different MP:RNA ratios. The 'thick string' complexes are suggested to represent clusters of MP molecules cooperatively bound to discrete regions of TMV RNA and separated by protein-free RNA segments.
引用
收藏
页码:1503 / 1508
页数:6
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