Rapid diagnosis of septic arthritis using 16S rDNA PCR: a comparison of 3 methods

被引：24

作者：

Bonilla, Hector ^{[2
]}

Kepley, Robert ^{[2
]}

Pawlak, Joan ^{[1
]}

Belian, Bradley ^{[1
]}

Raynor, Andrew ^{[2
]}

Saravolatz, Louis D. ^{[1
,3
]}

机构：

[1] St John Hosp & Med Ctr, Detroit, MI 48236 USA

[2] Summa Hlth Syst, Akron, OH 44304 USA

[3] Wayne State Univ, Sch Med, Detroit, MI 48201 USA

来源：

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2011年 / 69卷 / 04期

关键词：

Septic arthritis; Rapid diagnostic methods; PCR; POLYMERASE-CHAIN-REACTION; RIBOSOMAL-RNA GENE; JOINT INFECTIONS; DNA; AMPLIFICATION; IDENTIFICATION; FLUIDS; BONE;

D O I：

10.1016/j.diagmicrobio.2010.11.010

中图分类号：

R51 [传染病];

学科分类号：

100201 [内科学];

摘要：

Few studies address the utility of molecular techniques for diagnosis of infection in synovial fluid (SF). We evaluated 3 different methods using 16S rDNA polymerase chain reaction (PCR) on 63 specimens for the diagnosis of joint infection. SF samples were classified as normal, inflammatory, or septic based on the patient's clinical and laboratory results. Samples were analyzed by conventional PCR using primers for the bacterial 16S rDNA gene and by real-time PCR utilizing 2 different sets of primers for the target gene 16S rDNA. PCR results were compared to culture results. All inflammatory and normal SF samples were culture negative. There was concordance with 10 of the 16 septic samples by 2 of the PCR methods. When comparing 3 methods for rapid detection of septic arthritis, real-time PCR using SYBR-Green I and conventional PCR demonstrated favorable test characteristics, but need further study. (C) 2011 Elsevier Inc. All rights reserved.

引用

页码：390 / 395

页数：6

共 22 条

[1]

DNA can interfere with detection of Borrelia burgdorferi in skin biopsy specimens by PCR [J].