Feeding strategy of propionic acid for production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) with Ralstonia eutropha

被引:41
作者
Du, GCC
Chen, J
Yu, J
Lun, SY
机构
[1] Hong Kong Univ Sci & Technol, Dept Chem Engn, Kowloon, Hong Kong, Peoples R China
[2] Wuxi Univ Light Ind, Sch Biotechnol, Wuxi, Jiangsu, Peoples R China
关键词
Ralstonia eutropha; poly(3-hydroxybutyrate-co-3-hydroxyvalerate); poly-3-hydroxyalkanoates; feeding strategy; fed-batch culture; propionic acid;
D O I
10.1016/S1369-703X(01)00091-2
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The effects of propionic acid feeding strategy on production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) [P(3HB-co-3HV)] were studied with Ralstonia eutropha. Flask culture revealed that the time and concentration of propionic acid addition had significant effects on cell growth, P(3HB-co-3HV) synthesis, and HV fraction in the copolymer. In fed-batch culture, a low ratio of propionic acid to glucose (P/G) led to high dry cell weight (DCW), P(3HB-co-3HV) content and productivity, but low HV unit fraction. A high P/G ratio led to, on the other hand, high HV unit fraction but the low P(3HB-co-3HV) content and productivity. The specific P(3HB-co-3HV) synthetic rate and the specific HV synthetic rate declined in fed-batch cultures, which deteriorated with high P/G feeding due to the inhibitory effect of propionic acid accumulated in the culture broth. According to the non-steady state synthesis of P(3HB-co-3HV) by R. eutropha, an optimal feeding strategy to control the propionic acid accumulation was developed and demonstrated. The propionic acid feeding rate was reduced with time, and DCW, P(3HB-co-3HV) concentration, P(3HB-co-3HV) content and RV fraction in P(3HB-co-3HV) reached 52.1 gl(-1), 40.8 gl(-1), 78.3% and 16.2 mol%, respectively. P(3HB-co-3HV) productivity was 0.74 gl(-1) h(-1). (C) 2001 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:103 / 110
页数:8
相关论文
共 29 条
[11]  
DU GC, 1997, CHINESE J APPL ENV B, V3, P371
[12]   Poly(β-hydroxybutyric acid) thermoplastic production by Alcaligenes lotus:: Behavior of fed-batch cultures [J].
Grothe, E ;
Chisti, Y .
BIOPROCESS ENGINEERING, 2000, 22 (05) :441-449
[13]   ACCUMULATION OF A POLYHYDROXYALKANOATE CONTAINING PRIMARILY 3-HYDROXYDECANOATE FROM SIMPLE CARBOHYDRATE SUBSTRATES BY PSEUDOMONAS SP STRAIN NCIMB-40135 [J].
HAYWOOD, GW ;
ANDERSON, AJ ;
EWING, DF ;
DAWES, EA .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1990, 56 (11) :3354-3359
[14]  
HOLMES PA, 1990, DEV CRYSTALLINE POLY, V2, P1
[15]   Mole fraction control of poly(3-hydroxybutyric-co-3-hydroxyvaleric) acid in fed-batch culture of Alcaligenes eutrophus [J].
Ishihara, Y ;
Shimizu, H ;
Shioya, S .
JOURNAL OF FERMENTATION AND BIOENGINEERING, 1996, 81 (05) :422-428
[16]   PRODUCTION OF POLY(3-HYDROXYBUTYRIC-CO-3-HYDROXYVALERIC ACID) BY FED-BATCH CULTURE OF ALCALIGENES-EUTROPHUS WITH SUBSTRATE CONTROL USING ONLINE GLUCOSE ANALYZER [J].
KIM, BS ;
LEE, SC ;
LEE, SY ;
CHANG, HN ;
CHANG, YK ;
WOO, SI .
ENZYME AND MICROBIAL TECHNOLOGY, 1994, 16 (07) :556-561
[17]   EFFECT OF PROPIONIC-ACID ON POLY (BETA-HYDROXYBUTYRIC-CO-BETA-HYDROXYVALERIC) ACID PRODUCTION BY ALCALIGENES-EUTROPHUS [J].
KIM, JH ;
KIM, BG ;
CHOI, CY .
BIOTECHNOLOGY LETTERS, 1992, 14 (10) :903-906
[18]   EFFECTS OF PROPIONATE ON ACCUMULATION OF POLY(BETA-HYDROXYBUTYRATE-CO-BETA-HYDROXYVALERATE) AND EXCRETION OF PYRUVATE IN ALCALIGENES-EUTROPHUS [J].
LEE, IY ;
KIM, MK ;
CHANG, HN ;
PARK, YH .
BIOTECHNOLOGY LETTERS, 1994, 16 (06) :611-616
[19]   Plastic bacteria? Progress and prospects for polyhydroxyalkanoate production in bacteria [J].
Lee, SY .
TRENDS IN BIOTECHNOLOGY, 1996, 14 (11) :431-438
[20]  
LEE SY, 1996, BIOTECHNOL BIOENG, V43, P892