Photopolymerized sol-gel monoliths for capillary electrochromatography

被引：124

作者：

Dulay, MT ^{[1
]}

Quirino, JP ^{[1
]}

Bennett, BD ^{[1
]}

Kato, M ^{[1
]}

Zare, RN ^{[1
]}

机构：

[1] Stanford Univ, Dept Chem, Stanford, CA 94305 USA

来源：

ANALYTICAL CHEMISTRY | 2001年 / 73卷 / 16期

关键词：

D O I：

10.1021/ac0100749

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A solution of methactyloxypropylbrimethoxysilane in the presence of an acid catalyst, water, toluene, and a photoinitiator was irradiated at 365 nm for 5 min in a 75-mum i.d. capillary to prepare a porous monolithic sol-gel column by a one-step, in situ, process. The photopolymerized sol-gel (PSG) column shows reversed-phase behavior. Using this column, a variety of low-molecular-weight neutral compounds, including polycyclic aromatic hydrocarbons, alkyl benzenes, alkyl phenyl ketones, and steroids are separated from mixtures. Various different operational parameters, such as buffer composition, field strength, and column temperature, were varied to assess their influence on column performance. Use of PSG as a stationary phase for a pressure-driven separation is also demonstrated.

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收藏

页码：3921 / 3926

页数：6

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