Direct readout of protein-protein interactions by mass spectrometry from protein-DNA microarrays

被引：37

作者：

Becker, CFW

Wacker, R

Bouschen, W

Seidel, R

Kolaric, B

Lang, P

Schroeder, H

Müller, O

Niemeyer, CM

Spengler, B

Goody, RS

Engelhard, M

机构：

[1] Max Planck Inst Mol Physiol, D-44227 Dortmund, Germany

[2] Chimera Biotec GmbH, D-44227 Dortmund, Germany

[3] Univ Giessen, D-35392 Giessen, Germany

[4] Univ Dortmund, D-44227 Dortmund, Germany

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2005年 / 44卷 / 46期

关键词：

mass spectrometry; microarrays; oligonucleotides; protein-DNA conjugates; proteins;

D O I：

10.1002/anie.200502908

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

(Figure Presented) Catching proteins: Proteins from a protein-DNA microarray can be directly detected by using MALDI mass spectrometry. Capture proteins are attached to silicon chips in a highly selective and reversible way by DNA-directed immobilization (see picture). The analyte proteins bound specifically to their partners on the microarray can be detected from cell lysates. © 2005 Wiley-VCH Verlag GmbH & Co. KGaA.

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收藏

页码：7635 / 7639

页数：5

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