Crystallization of gankyrin, an oncoprotein that interacts with CDK4 and the S6b (rpt3) ATPase of the 19S regulator of the 26S proteasome

被引：9

作者：

Krzywda, S

Brzozowski, AM ^{[1
]}

Al-Safty, R

Welchman, R

Mee, M

Dawson, S

Fujita, J

Higashitsuji, H

Mayer, RJ

Wilkinson, AJ

机构：

[1] Univ York, Struct Biol Lab, Dept Chem, York YO10 5YW, N Yorkshire, England

[2] Univ Nottingham, Sch Med, Lab Intracellular Proteolysis, Sch Biomed Sci,Queens Med Ctr, Nottingham NG7 2UH, England

[3] Kyoto Univ, Dept Clin Mol Biol, Fac Med, Sakyo Ku, Kyoto 6068507, Japan

来源：

ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY | 2003年 / 59卷

关键词：

D O I：

10.1107/S0907444903009892

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Gankyrin is an oncoprotein overexpressed in hepatocarcinoma cells that binds to the cell-cycle regulator CDK4 and the S6b ATPase subunit of the regulatory component of the proteasome. It belongs to the family of ankyrin-repeat proteins that appear to mediate protein-protein interactions in diverse biochemical processes. Gankyrin has been crystallized from polyethylene glycol solutions and diffraction data have been obtained from these crystals that extend to 2.1 Angstrom spacing.

引用

页码：1294 / 1295

页数：2